1idf: Difference between revisions

Revision as of 19:52, 2 May 2008

ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME

OverviewOverview

Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized.

About this StructureAbout this Structure

1IDF is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase., Bolduc JM, Dyer DH, Scott WG, Singer P, Sweet RM, Koshland DE Jr, Stoddard BL, Science. 1995 Jun 2;268(5215):1312-8. PMID:7761851 Page seeded by OCA on Fri May 2 19:52:31 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1idf.jpg|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idf OCA], [http://www.ebi.ac.uk/pdbsum/1idf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1idf RCSB]</span>
-}}
 '''ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME'''
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 Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase., Bolduc JM, Dyer DH, Scott WG, Singer P, Sweet RM, Koshland DE Jr, Stoddard BL, Science. 1995 Jun 2;268(5215):1312-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7761851 7761851]
 [[Category: Escherichia coli]]
-[[Category: Isocitrate dehydrogenase (NADP(+))]]
 [[Category: Single protein]]
 [[Category: Bolduc, J M.]]
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 [[Category: Stoddard, B L.]]
 [[Category: Sweet, R M.]]
-[[Category: oxidoreductase (nad(a)-choh(d))]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:52:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:18:12 2008''