1ibh: Difference between revisions

Revision as of 19:48, 2 May 2008

X-RAY 3D STRUCTURE OF P.LEIOGNATHI CU,ZN SOD MUTANT M41I

OverviewOverview

The functional properties and X-ray structures of five mutant forms of Photobacterium leiognathi Cu,Zn superoxide dismutase carrying single mutations at residues located at the dimer association interface have been investigated. When compared to the wild-type enzyme, the three-dimensional structures of the mutants show structural perturbations limited to the proximity of the mutation sites and substantial identity of active site geometry. Nonetheless, the catalytic rates of all mutants, measured at neutral pH and low ionic strength by pulse radiolysis, are higher than that of the wild-type protein. Such enzymatic activity increase is paralleled by enhanced active site accessibility to external chelating agents, which, in the mutated enzyme, remove more readily the active site copper ion. It is concluded that mutations at the prokaryotic Cu,Zn superoxide dismutase subunit interface can transduce dynamical perturbation to the active site region, promoting substrate active site accessibility. Such long-range intramolecular communication effects have not been extensively described before within the Cu,Zn superoxide dismutase homology family.

About this StructureAbout this Structure

1IBH is a Single protein structure of sequence from Photobacterium leiognathi. Full crystallographic information is available from OCA.

ReferenceReference

Single mutations at the subunit interface modulate copper reactivity in Photobacterium leiognathi Cu,Zn superoxide dismutase., Stroppolo ME, Pesce A, D'Orazio M, O'Neill P, Bordo D, Rosano C, Milani M, Battistoni A, Bolognesi M, Desideri A, J Mol Biol. 2001 May 4;308(3):555-63. PMID:11327787 Page seeded by OCA on Fri May 2 19:48:27 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1ibh.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ibh |SIZE=350|CAPTION= <scene name='initialview01'>1ibh</scene>, resolution 2.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1ibh", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ibh|  PDB=1ibh  |  SCENE=  }}
-|RELATEDENTRY=[[1bzo|1BZO]], [[1ib5|1IB5]], [[1ibb|1IBB]], [[1ibd|1IBD]], [[1ibf|1IBF]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ibh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ibh OCA], [http://www.ebi.ac.uk/pdbsum/1ibh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ibh RCSB]</span>
-}}
 '''X-RAY 3D STRUCTURE OF P.LEIOGNATHI CU,ZN SOD MUTANT M41I'''
@@ Line 36: / Line 33: @@
 [[Category: Rosano, C.]]
 [[Category: Stroppolo, M E.]]
-[[Category: prokaryotic superoxide dismutase]]
+[[Category: Prokaryotic superoxide dismutase]]
-[[Category: subunit interaction]]
+[[Category: Subunit interaction]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:48:27 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:17:24 2008''