5jaf: Difference between revisions
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==LeuT Na+-free Return State, C2 form at pH 5== | |||
<StructureSection load='5jaf' size='340' side='right' caption='[[5jaf]], [[Resolution|resolution]] 3.02Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5jaf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JAF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JAF FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jae|5jae]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jaf OCA], [http://pdbe.org/5jaf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jaf RCSB], [http://www.ebi.ac.uk/pdbsum/5jaf PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial members of the neurotransmitter:sodium symporter (NSS) family perform Na(+)-dependent amino-acid uptake and extrude H(+) in return. Previous NSS structures represent intermediates of Na(+)/substrate binding or intracellular release, but not the inward-to-outward return transition. Here we report crystal structures of Aquifex aeolicus LeuT in an outward-oriented, Na(+)- and substrate-free state likely to be H(+)-occluded. We find a remarkable rotation of the conserved Leu25 into the empty substrate-binding pocket and rearrangements of the empty Na(+) sites. Mutational studies of the equivalent Leu99 in the human serotonin transporter show a critical role of this residue on the transport rate. Molecular dynamics simulations show that extracellular Na(+) is blocked unless Leu25 is rotated out of the substrate-binding pocket. We propose that Leu25 facilitates the inward-to-outward transition by compensating a Na(+)- and substrate-free state and acts as the gatekeeper for Na(+) binding that prevents leak in inward-outward return transitions. | |||
A conserved leucine occupies the empty substrate site of LeuT in the Na(+)-free return state.,Malinauskaite L, Said S, Sahin C, Grouleff J, Shahsavar A, Bjerregaard H, Noer P, Severinsen K, Boesen T, Schiott B, Sinning S, Nissen P Nat Commun. 2016 May 25;7:11673. doi: 10.1038/ncomms11673. PMID:27221344<ref>PMID:27221344</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5jaf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bjerregaard, H]] | |||
[[Category: Boesen, T]] | [[Category: Boesen, T]] | ||
[[Category: Grouleff, J]] | |||
[[Category: Malinauskaite, L]] | [[Category: Malinauskaite, L]] | ||
[[Category: | [[Category: Nissen, P]] | ||
[[Category: Noer, P]] | |||
[[Category: Sahin, C]] | |||
[[Category: Said, S]] | |||
[[Category: Schiott, B]] | [[Category: Schiott, B]] | ||
[[Category: Severinsen, K]] | [[Category: Severinsen, K]] | ||
[[Category: | [[Category: Shahsavar, A]] | ||
[[Category: | [[Category: Sinning, S]] | ||
[[Category: Amino acid transporter]] | |||
[[Category: Membrane protein]] | |||
[[Category: Neurotransmitter:sodium symporter family]] | |||
[[Category: Transport protein]] | |||