1hxh: Difference between revisions

Revision as of 19:19, 2 May 2008

COMAMONAS TESTOSTERONI 3BETA/17BETA HYDROXYSTEROID DEHYDROGENASE

OverviewOverview

The enzyme 3beta/17beta-hydroxysteroid dehydrogenase (3beta/17beta-HSD) is a steroid-inducible component of the Gram-negative bacterium Comamonas testosteroni. It catalyzes the reversible reduction/dehydrogenation of the oxo/beta-hydroxy groups at positions 3 and 17 of steroid compounds, including hormones and isobile acids. Crystallographic analysis at 1.2 A resolution reveals the enzyme to have nearly identical subunits that form a tetramer with 222 symmetry. This is one of the largest oligomeric structures refined at this resolution. The subunit consists of a monomer with a single-domain structure built around a seven-stranded beta-sheet flanked by six alpha-helices. The active site contains a Ser-Tyr-Lys triad, typical for short-chain dehydrogenases/reductases (SDR). Despite their highly diverse substrate specificities, SDR members show a close to identical folding pattern architectures and a common catalytic mechanism. In contrast to other SDR apostructures determined, the substrate binding loop is well-defined. Analysis of structure-activity relationships of catalytic cleft residues, docking analysis of substrates and inhibitors, and accessible surface analysis explains how 3beta/17beta-HSD accommodates steroid substrates of different conformations.

About this StructureAbout this Structure

1HXH is a Single protein structure of sequence from Comamonas testosteroni. Full crystallographic information is available from OCA.

ReferenceReference

Structure of bacterial 3beta/17beta-hydroxysteroid dehydrogenase at 1.2 A resolution: a model for multiple steroid recognition., Benach J, Filling C, Oppermann UC, Roversi P, Bricogne G, Berndt KD, Jornvall H, Ladenstein R, Biochemistry. 2002 Dec 17;41(50):14659-68. PMID:12475215 Page seeded by OCA on Fri May 2 19:19:47 2008

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OCA

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 [[Image:1hxh.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1hxh", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3(or_17)-beta-hydroxysteroid_dehydrogenase 3(or 17)-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.51 1.1.1.51] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hxh OCA], [http://www.ebi.ac.uk/pdbsum/1hxh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hxh RCSB]</span>
-}}
 '''COMAMONAS TESTOSTERONI 3BETA/17BETA HYDROXYSTEROID DEHYDROGENASE'''
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 ==Reference==
 Structure of bacterial 3beta/17beta-hydroxysteroid dehydrogenase at 1.2 A resolution: a model for multiple steroid recognition., Benach J, Filling C, Oppermann UC, Roversi P, Bricogne G, Berndt KD, Jornvall H, Ladenstein R, Biochemistry. 2002 Dec 17;41(50):14659-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12475215 12475215]
-[[Category: 3(or 17)-beta-hydroxysteroid dehydrogenase]]
 [[Category: Comamonas testosteroni]]
 [[Category: Single protein]]
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 [[Category: Oppermann, U C.T.]]
 [[Category: Roversi, P.]]
-[[Category: alpha-beta]]
+[[Category: Alpha-beta]]
-[[Category: rossmann fold]]
+[[Category: Rossmann fold]]
-[[Category: short-chain dehydrogenase]]
+[[Category: Short-chain dehydrogenase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:19:47 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:11:42 2008''