1hrt: Difference between revisions

Revision as of 19:10, 2 May 2008

THE STRUCTURE OF A COMPLEX OF BOVINE ALPHA-THROMBIN AND RECOMBINANT HIRUDIN AT 2.8 ANGSTROMS RESOLUTION

OverviewOverview

Crystals of the complex of bovine alpha-thrombin with recombinant hirudin variant 1 have space group C222(1) with cell constants a = 59.11, b = 102.62, and c = 143.26 A. The orientation and position of the thrombin component was determined by molecular replacement and the hirudin molecule was fit in 2 magnitude of Fo - magnitude of Fc electron density maps. The structure was refined by restrained least squares and simulated annealing to R = 0.161 at 2.8-A resolution. The binding of hirudin to thrombin is generally similar to that observed in the crystals of human thrombin-hirudin. Several differences in the interactions of the COOH-terminal polypeptide of hirudin, specifically of residues Asp-55h, Phe-56h, Glu-57h, and Glu-58h, and a few differences in the interactions of the hirudin core, specifically of residues Asp-5h, Ser-19h, and Asn-20h, with thrombin from human thrombin-hirudin suggest that there is some flexibility in the binding of these 2 molecules. Most of the residues in the 9 subsites that bind fibrinopeptide A7-16 to thrombin also interact with the NH2-terminal domain of hirudin. The S1 subsite is a notable exception in that only 1 of its 6 residues, namely Ser-214, interacts with hirudin. The only difference between human and bovine thrombins that appears to influence the binding of hirudin is the replacement of Lys-149E by an acidic glutamate in the bovine enzyme.

About this StructureAbout this Structure

1HRT is a Protein complex structure of sequences from Bos taurus and Hirudo medicinalis. Full crystallographic information is available from OCA.

ReferenceReference

The structure of a complex of bovine alpha-thrombin and recombinant hirudin at 2.8-A resolution., Vitali J, Martin PD, Malkowski MG, Robertson WD, Lazar JB, Winant RC, Johnson PH, Edwards BF, J Biol Chem. 1992 Sep 5;267(25):17670-8. PMID:1517214 Page seeded by OCA on Fri May 2 19:10:15 2008

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OCA

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 [[Image:1hrt.gif|left|200px]]
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 '''THE STRUCTURE OF A COMPLEX OF BOVINE ALPHA-THROMBIN AND RECOMBINANT HIRUDIN AT 2.8 ANGSTROMS RESOLUTION'''
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 [[Category: Edwards, B F.P.]]
 [[Category: Vitali, J.]]
-[[Category: hydrolase(serine proteinase)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:10:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:09:36 2008''