5ipv: Difference between revisions
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<StructureSection load='5ipv' size='340' side='right' caption='[[5ipv]], [[Resolution|resolution]] 9.25Å' scene=''> | <StructureSection load='5ipv' size='340' side='right' caption='[[5ipv]], [[Resolution|resolution]] 9.25Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5ipv]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IPV FirstGlance]. <br> | <table><tr><td colspan='2'>[[5ipv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IPV FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5iou|5iou]], [[5iov|5iov]], [[5ipq|5ipq]], [[5ipr|5ipr]], [[5ips|5ips]], [[5ipt|5ipt]], [[5ipu|5ipu]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5iou|5iou]], [[5iov|5iov]], [[5ipq|5ipq]], [[5ipr|5ipr]], [[5ips|5ips]], [[5ipt|5ipt]], [[5ipu|5ipu]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ipv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ipv OCA], [http://pdbe.org/5ipv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ipv RCSB], [http://www.ebi.ac.uk/pdbsum/5ipv PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ipv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ipv OCA], [http://pdbe.org/5ipv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ipv RCSB], [http://www.ebi.ac.uk/pdbsum/5ipv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ipv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: African clawed frog]] | |||
[[Category: Goehring, A]] | [[Category: Goehring, A]] | ||
[[Category: Gouaux, E]] | [[Category: Gouaux, E]] | ||
Revision as of 15:48, 16 November 2017
Cryo-EM structure of GluN1/GluN2B NMDA receptor in the DCKA/D-APV-bound conformation, state 1Cryo-EM structure of GluN1/GluN2B NMDA receptor in the DCKA/D-APV-bound conformation, state 1
Structural highlights
Publication Abstract from PubMedN-methyl-D-aspartate receptors (NMDARs) are glutamate-gated, calcium-permeable ion channels that mediate synaptic transmission and underpin learning and memory. NMDAR dysfunction is directly implicated in diseases ranging from seizure to ischemia. Despite its fundamental importance, little is known about how the NMDAR transitions between inactive and active states and how small molecules inhibit or activate ion channel gating. Here, we report electron cryo-microscopy structures of the GluN1-GluN2B NMDA receptor in an ensemble of competitive antagonist-bound states, an agonist-bound form, and a state bound with agonists and the allosteric inhibitor Ro25-6981. Together with double electron-electron resonance experiments, we show how competitive antagonists rupture the ligand binding domain (LBD) gating "ring," how agonists retain the ring in a dimer-of-dimers configuration, and how allosteric inhibitors, acting within the amino terminal domain, further stabilize the LBD layer. These studies illuminate how the LBD gating ring is fundamental to signal transduction and gating in NMDARs. Mechanism of NMDA Receptor Inhibition and Activation.,Zhu S, Stein RA, Yoshioka C, Lee CH, Goehring A, Mchaourab HS, Gouaux E Cell. 2016 Apr 21;165(3):704-14. doi: 10.1016/j.cell.2016.03.028. Epub 2016 Apr, 7. PMID:27062927[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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