1hl7: Difference between revisions

Revision as of 18:58, 2 May 2008

GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE

OverviewOverview

The structure of the recombinant (-) gamma-lactamase from an Aureobacterium species has been solved at 1.73A resolution in the cubic space group F23 with unit cell parameters a=b=c=240.6A. The trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. The structure has been solved in complex with a covalently bound ligand originating from the host cell and also in the unligated form. The associated density in the former structure has been interpreted as the two-ring ligand (3aR,7aS)-3a,4,7,7a-tetrahydro-benzo [1,3] dioxol-2-one which forms a tetrahedral complex with OG of the catalytic Ser98. Soaks of these crystals with the industrial substrate gamma-lactam or its structural analogue, norcamphor, result in the displacement of the ligand from the enzyme active site, thereby allowing determination of the unligated structure. The presence of the ligand in the active site protects the enzyme from serine hydrolase inhibitors. Cyclic ethylene carbonate, the first ring of the ligand, was shown to be a substrate of the enzyme.

About this StructureAbout this Structure

1HL7 is a Single protein structure of sequence from Microbacterium. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site., Line K, Isupov MN, Littlechild JA, J Mol Biol. 2004 Apr 30;338(3):519-32. PMID:15081810 Page seeded by OCA on Fri May 2 18:58:19 2008

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OCA

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 [[Image:1hl7.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1hl7 |SIZE=350|CAPTION= <scene name='initialview01'>1hl7</scene>, resolution 1.73&Aring;
+The line below this paragraph, containing "STRUCTURE_1hl7", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Bd1+Binding+Site+For+Chain+B'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BD1:3A,4,7,7A-TETRAHYDRO-BENZO+[1,3]+DIOXOL-2-ONE'>BD1</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1hl7|  PDB=1hl7  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hl7 OCA], [http://www.ebi.ac.uk/pdbsum/1hl7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hl7 RCSB]</span>
-}}
 '''GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE'''
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 [[Category: Line, K.]]
 [[Category: Littlechild, J A.]]
-[[Category: alpha/beta hydrolase]]
+[[Category: Alpha/beta hydrolase]]
-[[Category: co-factor free haloperoxidase]]
+[[Category: Co-factor free haloperoxidase]]
-[[Category: tetrahedral intermediate]]
+[[Category: Tetrahedral intermediate]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:58:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:07:04 2008''