1hj8: Difference between revisions

Revision as of 18:54, 2 May 2008

1.00 AA TRYPSIN FROM ATLANTIC SALMON

OverviewOverview

Radiation damage is an inherent problem in protein X-ray crystallography and the process has recently been shown to be highly specific, exhibiting features such as cleavage of disulfide bonds, decarboxylation of acidic residues, increase in atomic B factors and increase in unit-cell volume. Reported here are two trypsin structures at atomic resolution (1.00 and 0.95 A), the data for which were collected at a third-generation synchrotron (ESRF) at two different beamlines. Both trypsin structures exhibit broken disulfide bonds; in particular, the bond from Cys191 to Cys220 is very sensitive to synchrotron radiation. The data set collected at the most intense beamline (ID14-EH4) shows increased structural radiation damage in terms of lower occupancies for cysteine residues, more breakage in the six disulfide bonds and more alternate conformations. It appears that high intensity and not only the total X-ray dose is most harmful to protein crystals.

About this StructureAbout this Structure

1HJ8 is a Single protein structure of sequence from Salmo salar. Full crystallographic information is available from OCA.

ReferenceReference

Atomic resolution structures of trypsin provide insight into structural radiation damage., Leiros HK, McSweeney SM, Smalas AO, Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):488-97. PMID:11264577 Page seeded by OCA on Fri May 2 18:53:58 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1hj8.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1hj8 |SIZE=350|CAPTION= <scene name='initialview01'>1hj8</scene>, resolution 1.00&Aring;
+The line below this paragraph, containing "STRUCTURE_1hj8", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=CA1:Bam+Binding+Site+For+Residue+A248'>CA1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BAM:BENZAMIDINE'>BAM</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1hj8|  PDB=1hj8  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hj8 OCA], [http://www.ebi.ac.uk/pdbsum/1hj8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hj8 RCSB]</span>
-}}
 '''1.00 AA TRYPSIN FROM ATLANTIC SALMON'''
@@ Line 29: / Line 26: @@
 [[Category: Mcsweeney, S M.]]
 [[Category: Smalas, A O.]]
-[[Category: atomic resolution]]
+[[Category: Atomic resolution]]
-[[Category: disulphide bond breakage]]
+[[Category: Disulphide bond breakage]]
-[[Category: radiation damage]]
+[[Category: Radiation damage]]
-[[Category: salmon]]
+[[Category: Salmon]]
-[[Category: trypsin]]
+[[Category: Trypsin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:53:58 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:06:03 2008''