1hi5: Difference between revisions
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'''EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX''' | '''EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX''' | ||
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[[Category: Turton, R.]] | [[Category: Turton, R.]] | ||
[[Category: Youle, R J.]] | [[Category: Youle, R J.]] | ||
[[Category: | [[Category: Ribonuclease]] | ||
[[Category: | [[Category: Rnase us]] | ||
[[Category: | [[Category: Rnase-2]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:52:10 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 18:52, 2 May 2008
EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX
OverviewOverview
Eosinophil-derived neurotoxin (EDN), a basic ribonuclease found in the large specific granules of eosinophils, belongs to the pancreatic RNase A family. Although its physiological function is still unclear, it has been shown that EDN is a neurotoxin capable of inducing the Gordon phenomenon in rabbits. EDN is also a potent helminthotoxin and can mediate antiviral activity of eosinophils against isolated virions of the respiratory syncytial virus. EDN is a catalytically efficient RNase sharing similar substrate specificity with pancreatic RNase A with its ribonucleolytic activity being absolutely essential for its neurotoxic, helminthotoxic, and antiviral activities. The crystal structure of recombinant human EDN in the unliganded form has been determined previously (Mosimann, S. C., Newton, D. L., Youle, R. J., and James, M. N. G. (1996) J. Mol. Biol. 260, 540-552). We have now determined high resolution (1.8 A) crystal structures for EDN in complex with adenosine-3',5'-diphosphate (3',5'-ADP), adenosine-2',5'-di-phosphate (2',5'-ADP), adenosine-5'-diphosphate (5'-ADP) as well as for a native structure in the presence of sulfate refined at 1.6 A. The inhibition constant of these mononucleotides for EDN has been determined. The structures present the first detailed picture of differences between EDN and RNase A in substrate recognition at the ribonucleolytic active site. They also provide a starting point for the design of tight-binding inhibitors, which may be used to restrain the RNase activity of EDN.
About this StructureAbout this Structure
1HI5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors., Leonidas DD, Boix E, Prill R, Suzuki M, Turton R, Minson K, Swaminathan GJ, Youle RJ, Acharya KR, J Biol Chem. 2001 May 4;276(18):15009-17. Epub 2001 Jan 11. PMID:11154698 Page seeded by OCA on Fri May 2 18:52:10 2008