1hi0: Difference between revisions

Revision as of 18:51, 2 May 2008

RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS INITIATION COMPLEX

OverviewOverview

In most RNA viruses, genome replication and transcription are catalysed by a viral RNA-dependent RNA polymerase. Double-stranded RNA viruses perform these operations in a capsid (the polymerase complex), using an enzyme that can read both single- and double-stranded RNA. Structures have been solved for such viral capsids, but they do not resolve the polymerase subunits in any detail. Here we show that the 2 A resolution X-ray structure of the active polymerase subunit from the double-stranded RNA bacteriophage straight phi6 is highly similar to that of the polymerase of hepatitis C virus, providing an evolutionary link between double-stranded RNA viruses and flaviviruses. By crystal soaking and co-crystallization, we determined a number of other structures, including complexes with oligonucleotide and/or nucleoside triphosphates (NTPs), that suggest a mechanism by which the incoming double-stranded RNA is opened up to feed the template through to the active site, while the substrates enter by another route. The template strand initially overshoots, locking into a specificity pocket, and then, in the presence of cognate NTPs, reverses to form the initiation complex; this process engages two NTPs, one of which acts with the carboxy-terminal domain of the protein to prime the reaction. Our results provide a working model for the initiation of replication and transcription.

About this StructureAbout this Structure

1HI0 is a Single protein structure of sequence from Pseudomonas phage phi6. Full crystallographic information is available from OCA.

ReferenceReference

A mechanism for initiating RNA-dependent RNA polymerization., Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI, Nature. 2001 Mar 8;410(6825):235-40. PMID:11242087 Page seeded by OCA on Fri May 2 18:51:55 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1hi0.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1hi0 |SIZE=350|CAPTION= <scene name='initialview01'>1hi0</scene>, resolution 3.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1hi0", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=CA1:The+Three+Conserved+Active+Site+Aspartate'>CA1</scene>, <scene name='pdbsite=CA2:The+Three+Conserved+Active+Site+Aspartate'>CA2</scene>, <scene name='pdbsite=CA3:The+Three+Conserved+Active+Site+Aspartate'>CA3</scene>, <scene name='pdbsite=GP1:Gtp+Binding+Site+For+Residue+P666'>GP1</scene>, <scene name='pdbsite=GP2:Gtp+Binding+Site+For+Residue+P667'>GP2</scene>, <scene name='pdbsite=GQ1:Gtp+Binding+Site+For+Residue+Q666'>GQ1</scene>, <scene name='pdbsite=GQ2:Gtp+Binding+Site+For+Residue+Q667'>GQ2</scene>, <scene name='pdbsite=GR1:Gtp+Binding+Site+For+Residue+R666'>GR1</scene> and <scene name='pdbsite=GR2:Gtp+Binding+Site+For+Residue+R667'>GR2</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1hi0|  PDB=1hi0  |  SCENE=  }}
-|RELATEDENTRY=[[1hhs|1HHS]], [[1hht|1HHT]], [[1hi1|1HI1]], [[1hi8|1HI8]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hi0 OCA], [http://www.ebi.ac.uk/pdbsum/1hi0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hi0 RCSB]</span>
-}}
 '''RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS INITIATION COMPLEX'''
@@ Line 30: / Line 27: @@
 [[Category: Makeyev, E V.]]
 [[Category: Stuart, D I.]]
-[[Category: rna polymerase]]
+[[Category: Rna polymerase]]
-[[Category: viral polymerase]]
+[[Category: Viral polymerase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:51:55 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:05:21 2008''