1h3f: Difference between revisions

Revision as of 18:23, 2 May 2008

TYROSYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH TYROSINOL

OverviewOverview

Bacterial tyrosyl-tRNA synthetases (TyrRS) possess a flexibly linked C-terminal domain of approximately 80 residues, which has hitherto been disordered in crystal structures of the enzyme. We have determined the structure of Thermus thermophilus TyrRS at 2.0 A resolution in a crystal form in which the C-terminal domain is ordered, and confirm that the fold is similar to part of the C-terminal domain of ribosomal protein S4. We have also determined the structure at 2.9 A resolution of the complex of T.thermophilus TyrRS with cognate tRNA(tyr)(G Psi A). In this structure, the C-terminal domain binds between the characteristic long variable arm of the tRNA and the anti-codon stem, thus recognizing the unique shape of the tRNA. The anticodon bases have a novel conformation with A-36 stacked on G-34, and both G-34 and Psi-35 are base-specifically recognized. The tRNA binds across the two subunits of the dimeric enzyme and, remarkably, the mode of recognition of the class I TyrRS for its cognate tRNA resembles that of a class II synthetase in being from the major groove side of the acceptor stem.

About this StructureAbout this Structure

1H3F is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Class I tyrosyl-tRNA synthetase has a class II mode of cognate tRNA recognition., Yaremchuk A, Kriklivyi I, Tukalo M, Cusack S, EMBO J. 2002 Jul 15;21(14):3829-40. PMID:12110594 Page seeded by OCA on Fri May 2 18:23:07 2008

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OCA

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 [[Image:1h3f.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1h3f |SIZE=350|CAPTION= <scene name='initialview01'>1h3f</scene>, resolution 2.00&Aring;
+The line below this paragraph, containing "STRUCTURE_1h3f", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Tyb+Binding+Site+For+Chain+B'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TYB:TYROSINAL'>TYB</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1h3f|  PDB=1h3f  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h3f OCA], [http://www.ebi.ac.uk/pdbsum/1h3f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h3f RCSB]</span>
-}}
 '''TYROSYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH TYROSINOL'''
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 [[Category: Tukalo, M.]]
 [[Category: Yaremchuk, A.]]
-[[Category: aminoacyl-trna synthetase]]
+[[Category: Aminoacyl-trna synthetase]]
-[[Category: atp + l-tyrosine + trna(tyr)->amp + ppi + l-tyrosyl-trna(ty class i aminoacyl-trna synthetase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:23:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:56:53 2008''