1gu0: Difference between revisions

Revision as of 18:00, 2 May 2008

CRYSTAL STRUCTURE OF TYPE II DEHYDROQUINASE FROM STREPTOMYCES COELICOLOR

OverviewOverview

The structure of the type II DHQase from Streptomyces coelicolor has been solved and refined to high resolution in complexes with a number of ligands, including dehydroshikimate and a rationally designed transition state analogue, 2,3-anhydro-quinic acid. These structures define the active site of the enzyme and the role of key amino acid residues and provide snap shots of the catalytic cycle. The resolution of the flexible lid domain (residues 21-31) shows that the invariant residues Arg23 and Tyr28 close over the active site cleft. The tyrosine acts as the base in the initial proton abstraction, and evidence is provided that the reaction proceeds via an enol intermediate. The active site of the structure of DHQase in complex with the transition state analog also includes molecules of tartrate and glycerol, which provide a basis for further inhibitor design.

About this StructureAbout this Structure

1GU0 is a Single protein structure of sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA.

ReferenceReference

The structure and mechanism of the type II dehydroquinase from Streptomyces coelicolor., Roszak AW, Robinson DA, Krell T, Hunter IS, Fredrickson M, Abell C, Coggins JR, Lapthorn AJ, Structure. 2002 Apr;10(4):493-503. PMID:11937054 Page seeded by OCA on Fri May 2 18:00:20 2008

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OCA

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 [[Image:1gu0.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1gu0", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-dehydroquinate_dehydratase 3-dehydroquinate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.10 4.2.1.10] </span>
+or leave the SCENE parameter empty for the default display.
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+-->
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gu0 OCA], [http://www.ebi.ac.uk/pdbsum/1gu0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gu0 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF TYPE II DEHYDROQUINASE FROM STREPTOMYCES COELICOLOR'''
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 [[Category: Robinson, D.]]
 [[Category: Roszak, A W.]]
-[[Category: dodecameric quaternary structure]]
+[[Category: Dodecameric quaternary structure]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: shikimate pathway]]
+[[Category: Shikimate pathway]]
-[[Category: tetrahedral symmetry]]
+[[Category: Tetrahedral symmetry]]
-[[Category: type ii dehydroquinase]]
+[[Category: Type ii dehydroquinase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:00:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:51:14 2008''