1gqv: Difference between revisions

Revision as of 17:54, 2 May 2008

ATOMIC RESOLUTION (0.98A) STRUCTURE OF EOSINOPHIL-DERIVED NEUROTOXIN

OverviewOverview

Human eosinophil-derived neurotoxin (EDN) is a small, basic protein that belongs to the ribonuclease A superfamily. EDN displays antiviral activity and causes the neurotoxic Gordon phenomenon when injected into rabbits. Although EDN and ribonuclease A have appreciable structural similarity and a conserved catalytic triad, their peripheral substrate-binding sites are not conserved. The crystal structure of recombinant EDN (rEDN) has been determined at 0.98 A resolution from data collected at a low temperature (100 K). We have refined the crystallographic model of the structure using anisotropic displacement parameters to a conventional R-factor of 0.116. This represents the highest resolution structure of rEDN determined to date and is only the second ribonuclease structure to be determined at a resolution greater than 1.0 A. The structure provides a detailed picture of the conformational freedom at the various subsites of rEDN, and the water structure accounts for more than 50% of the total solvent content of the unit cell. This information will be crucial for the design of tight-binding inhibitors to restrain the ribonucleolytic activity of rEDN.

About this StructureAbout this Structure

1GQV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Atomic resolution (0.98 A) structure of eosinophil-derived neurotoxin., Swaminathan GJ, Holloway DE, Veluraja K, Acharya KR, Biochemistry. 2002 Mar 12;41(10):3341-52. PMID:11876642 Page seeded by OCA on Fri May 2 17:54:33 2008

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OCA

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 [[Image:1gqv.jpg|left|200px]]
- {{Structure
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-}}
 '''ATOMIC RESOLUTION (0.98A) STRUCTURE OF EOSINOPHIL-DERIVED NEUROTOXIN'''
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 [[Category: Swaminathan, G J.]]
 [[Category: Veluraja, K.]]
-[[Category: ribonuclease]]
+[[Category: Ribonuclease]]
-[[Category: rnase us]]
+[[Category: Rnase us]]
-[[Category: rnase-2]]
+[[Category: Rnase-2]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:54:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:49:43 2008''