Glutamate synthase: Difference between revisions
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<StructureSection load='1llw' size='340' side='right' caption='Ferredoxin-dependent glutamate synthase containing a Fe3-S4 cluster complex with FMN, 2-oxo-glutarate (PDB code [[1llw]])' scene=''> | <StructureSection load='1llw' size='340' side='right' caption='Ferredoxin-dependent glutamate synthase containing a Fe3-S4 cluster complex with FMN, 2-oxo-glutarate (PDB code [[1llw]])' scene=''> | ||
== Function == | == Function == | ||
'''Glutamate synthase''' (GS) catalyzes the reverse reaction which converts L-glutamine, 2- | '''Glutamate synthase''' (GS) is an iron-sulfur flavoprotein which catalyzes the reverse reaction which converts L-glutamine, 2-oxoglutarate and NADPH to L-glutarate and NADP. | ||
<ref>PMID:18421771</ref>. Fd-dependent glutamate synthase catalyzes the reverse reaction converting L-glutamate and oxidized ferredoxin to L-glutamine, 2- | <ref>PMID:18421771</ref>. Fd-dependent glutamate synthase catalyzes the reverse reaction converting L-glutamate and oxidized ferredoxin to L-glutamine, 2-oxoglutarate and oxidized ferredoxin<ref>PMID:6746604</ref>. Fd-GS uses FMN as a cofactor. | ||
== Disease == | == Disease == | ||
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== Structural highlights == | == Structural highlights == | ||
The Fd-GS structure contains 4 domains. The N-terminal domain is an amidotransferase domain and contains an active site where residue 1Cys catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which contains an Fe3S4 cluster and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. Residue M475 is located between the FMN and the Fe3S4 cluster. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>. | |||
</StructureSection> | </StructureSection> | ||
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*Glutamate synthase | *Glutamate synthase | ||
**[[1ea0]] – AbGS | **[[1ea0]] – AbGS α subunit + FMN + oxo-glutarate – ''Azospirillum brasilense''<br /> | ||
**[[2vdc]] – AbGS | **[[2vdc]] – AbGS α+β subunits + FAD + FMN + oxoglutarate – CryoEM<br /> | ||
*Fd-dependent glutamate synthase | *Fd-dependent glutamate synthase | ||
**[[1llw]], [[1ofd]] – SyGS + FMN + | **[[1llw]], [[1ofd]] – SyGS + FMN + 2-αoxoglutarate – ''Synechocystis''<br /> | ||
**[[1llz]], [[1lm1]] – SyGS + FMN <br /> | **[[1llz]], [[1lm1]] – SyGS + FMN <br /> | ||
**[[1ofe]] – SyGS + FMN + oxglutarate + oxo-norleucine<br /> | **[[1ofe]] – SyGS + FMN + 2-oxglutarate + oxo-norleucine<br /> | ||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||