5a51: Difference between revisions
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==The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and phophatidyl serine== | ==The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and phophatidyl serine== | ||
<StructureSection load='5a51' size='340' side='right' caption='[[5a51]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='5a51' size='340' side='right'caption='[[5a51]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5a51]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A51 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5a51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A51 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a4x|5a4x]], [[5a50|5a50]], [[5a52|5a52]], [[4v29|4v29]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a4x|5a4x]], [[5a50|5a50]], [[5a52|5a52]], [[4v29|4v29]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a51 OCA], [http://pdbe.org/5a51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a51 RCSB], [http://www.ebi.ac.uk/pdbsum/5a51 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a51 OCA], [http://pdbe.org/5a51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a51 RCSB], [http://www.ebi.ac.uk/pdbsum/5a51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a51 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Arath]] | |||
[[Category: Large Structures]] | |||
[[Category: Albert, A]] | [[Category: Albert, A]] | ||
[[Category: Diaz, M]] | [[Category: Diaz, M]] | ||
Revision as of 11:48, 26 June 2019
The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and phophatidyl serineThe crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and phophatidyl serine
Structural highlights
Publication Abstract from PubMedRegulation of ion transport in plants is essential for cell function. Abiotic stress unbalances cell ion homeostasis, and plants tend to readjust it, regulating membrane transporters and channels. The plant hormone abscisic acid (ABA) and the second messenger Ca(2+) are central in such processes, as they are involved in the regulation of protein kinases and phosphatases that control ion transport activity in response to environmental stimuli. The identification and characterization of the molecular mechanisms underlying the effect of ABA and Ca(2+) signaling pathways on membrane function are central and could provide opportunities for crop improvement. The C2-domain ABA-related (CAR) family of small proteins is involved in the Ca(2+)-dependent recruitment of the pyrabactin resistance 1/PYR1-like (PYR/PYL) ABA receptors to the membrane. However, to fully understand CAR function, it is necessary to define a molecular mechanism that integrates Ca(2+) sensing, membrane interaction, and the recognition of the PYR/PYL interacting partners. We present structural and biochemical data showing that CARs are peripheral membrane proteins that functionally cluster on the membrane and generate strong positive membrane curvature in a Ca(2+)-dependent manner. These features represent a mechanism for the generation, stabilization, and/or specific recognition of membrane discontinuities. Such structures may act as signaling platforms involved in the recruitment of PYR/PYL receptors and other signaling components involved in cell responses to stress. Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling.,Diaz M, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez-Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez PL, Albert A Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):E396-405. doi:, 10.1073/pnas.1512779113. Epub 2015 Dec 30. PMID:26719420[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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