1gh9: Difference between revisions

Revision as of 17:33, 2 May 2008

SOLUTION STRUCTURE OF A 8.3 KDA PROTEIN (GENE MTH1184) FROM METHANOBACTERIUM THERMOAUTOTROPHICUM

OverviewOverview

A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, approximately 20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome. A retrospective analysis of the experimental behavior of these proteins suggested some simple relations between sequence and solubility, implying that data bases of protein properties will be useful in optimizing high throughput strategies. Of the first 10 structures determined, several provided clues to biochemical functions that were not detectable from sequence analysis, and in many cases these putative functions could be readily confirmed by biochemical methods. This demonstrates that structural proteomics is feasible and can play a central role in functional genomics.

About this StructureAbout this Structure

1GH9 is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. This structure supersedes the now removed PDB entry 1dw7. Full crystallographic information is available from OCA.

ReferenceReference

Structural proteomics of an archaeon., Christendat D, Yee A, Dharamsi A, Kluger Y, Savchenko A, Cort JR, Booth V, Mackereth CD, Saridakis V, Ekiel I, Kozlov G, Maxwell KL, Wu N, McIntosh LP, Gehring K, Kennedy MA, Davidson AR, Pai EF, Gerstein M, Edwards AM, Arrowsmith CH, Nat Struct Biol. 2000 Oct;7(10):903-9. PMID:11017201 Page seeded by OCA on Fri May 2 17:33:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1gh9.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1gh9", creates the "Structure Box" on the page.
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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-|GENE= MTH1184 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam09082 DUF1922]</span>
+{{STRUCTURE_1gh9|  PDB=1gh9  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gh9 OCA], [http://www.ebi.ac.uk/pdbsum/1gh9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gh9 RCSB]</span>
-}}
 '''SOLUTION STRUCTURE OF A 8.3 KDA PROTEIN (GENE MTH1184) FROM METHANOBACTERIUM THERMOAUTOTROPHICUM'''
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 ==About this Structure==
-GH9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. This structure supersedes the now removed PDB entry 1DW7. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GH9 OCA].
+GH9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1dw7 1dw7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GH9 OCA].
 ==Reference==
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 [[Category: Kozlov, G.]]
 [[Category: NESG, Northeast Structural Genomics Consortium.]]
-[[Category: beta+alpha complex structure]]
+[[Category: Beta+alpha complex structure]]
-[[Category: nesg]]
+[[Category: Nesg]]
-[[Category: northeast structural genomics consortium]]
+[[Category: Northeast structural genomics consortium]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:33:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:43:55 2008''