Z-DNA model tour

Z-form DNA modelZ-form DNA model

Structural highlights Helix has left-handed sense Can be formed in vivo, given proper sequence and superhelical tension, but function remains obscure. Narrower, more elongated helix than A or B. Major "groove" not really groove Narrow minor groove Conformation favored by high salt concentrations, some base substitutions, but requires alternating purine-pyrimidine sequence. Base pairs nearly perpendicular to helix axis GpC repeat, not single base-pair P-P distances: vary for GpC and CpG GpC stack: good base overlap CpG: less overlap. Zigzag backbone due to C sugar conformation compensating for G glycosidic bond conformation Conformations: G; syn, C2'-endo C; anti, C3'-endo Take the Tour The tour starts with the view. Now look at this .The backbone is yellow and the bases are magenta. Note that the major groove (in the middle, when you have just clicked the button) is wide and easily accessible. Now change the display to make it show the connecting the phosphate atoms. Now the bases are easier to see. Notice how they are stacked upon each other and are nearly perpendicular to the axis of the double helix. Note also that the backbone forms a smooth, continuous curve. You can .You are looking into the major groove and the colors of the base pairs alternate. You can also . Each base pair stacks on the next similarly, as shown from . This is the . A-form DNA also stacks in this way, but compare this with Z-DNA, which behaves much differently. DNA is usually found in the B form under physiological conditions. The B-form conformation is stabilized by water molecules bound to the minor groove. You can see them as red dots . Sometimes kinks are found in the B helix at transcriptional control regions. These kinks can either be intrinsic to the DNA sequence or caused by transcription factor binding. You can compare it with the DNA forms by looking at this 3D red-blue stern picture of A, B, and Z DNA

spinqualitylabelsall models B-DNA Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

ReferencesReferences

R. E. Dickerson, H. R. Drew, B. N. Conner, R. M. Wing, A. V. Fratini & M. L. Kopka (1982) The anatomy of A-, B-, and Z-DNA. Science 216: 475-485 JSmol in Proteopedia or to the article describing Jmol to the rescue.