1g71: Difference between revisions

Revision as of 17:13, 2 May 2008

CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE

OverviewOverview

Primases are essential components of the DNA replication apparatus in every organism. They catalyze the synthesis of oligoribonucleotides on single-stranded DNA, which subsequently serve as primers for the replicative DNA polymerases. In contrast to bacterial primases, the archaeal enzymes are closely related to their eukaryotic counterparts. We have solved the crystal structure of the catalytic primase subunit from the hyperthermophilic archaeon Pyrococcus furiosus at 2.3 A resolution by multiwavelength anomalous dispersion methods. The structure shows a two-domain arrangement with a novel zinc knuckle motif located in the primase (prim) domain. In this first structure of a complete protein of the archaeal/eukaryotic primase family, the arrangement of the catalytically active residues resembles the active sites of various DNA polymerases that are unrelated in fold.

About this StructureAbout this Structure

1G71 is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a DNA-dependent RNA polymerase (DNA primase)., Augustin MA, Huber R, Kaiser JT, Nat Struct Biol. 2001 Jan;8(1):57-61. PMID:11135672 Page seeded by OCA on Fri May 2 17:13:17 2008

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OCA

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 [[Image:1g71.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g71 OCA], [http://www.ebi.ac.uk/pdbsum/1g71 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g71 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE'''
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 [[Category: Huber, R.]]
 [[Category: Kaiser, J T.]]
-[[Category: zinc-knuckle]]
+[[Category: Zinc-knuckle]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:13:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:37:57 2008''