1g50: Difference between revisions
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'''CRYSTAL STRUCTURE OF A WILD TYPE HER ALPHA LBD AT 2.9 ANGSTROM RESOLUTION''' | '''CRYSTAL STRUCTURE OF A WILD TYPE HER ALPHA LBD AT 2.9 ANGSTROM RESOLUTION''' | ||
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[[Category: Ruff, M.]] | [[Category: Ruff, M.]] | ||
[[Category: SPINE, Structural Proteomics in Europe.]] | [[Category: SPINE, Structural Proteomics in Europe.]] | ||
[[Category: | [[Category: Alpha helice]] | ||
[[Category: | [[Category: Estradiol]] | ||
[[Category: | [[Category: Spine]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: | [[Category: Structural proteomics in europe]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:06:43 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun | |||
Revision as of 08:06, 13 April 2008
CRYSTAL STRUCTURE OF A WILD TYPE HER ALPHA LBD AT 2.9 ANGSTROM RESOLUTION
OverviewOverview
Several crystal structures of human estrogen receptor alpha ligand-binding domain (hERalpha LBD) complexed with agonist or antagonist molecules have previously been solved. The proteins had been modified in cysteine residues (carboxymethylation) or renatured in urea to circumvent aggregation and denaturation problems. In this work, high-level protein expression and purification together with crystallization screening procedure yielded high amounts of soluble protein without renaturation or modifications steps. The native protein crystallizes in the space group P3(2) 21 with three molecules in the asymmetric unit. The overall structure is very similar to that previously reported for the hERalpha LBD with cysteine carboxymethylated residues thus validating the modification approach. The present strategy can be adapted to other cases where the solubility and the proper folding is a difficulty.
About this StructureAbout this Structure
1G50 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Overexpression, purification, and crystal structure of native ER alpha LBD., Eiler S, Gangloff M, Duclaud S, Moras D, Ruff M, Protein Expr Purif. 2001 Jul;22(2):165-73. PMID:11437591 Page seeded by OCA on Sun Apr 13 08:06:43 2008