1f7j: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F7J FirstGlance]. <br> | <table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F7J FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f7j FirstGlance], [http://www.ebi.ac.uk/pdbsum/1f7j PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f7j FirstGlance], [http://www.ebi.ac.uk/pdbsum/1f7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1f7j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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Revision as of 12:20, 27 December 2017
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PL.LSU/2 GROUPII INTRONPL.LSU/2 GROUPII INTRON
Structural highlights
Publication Abstract from PubMedWe have used chemical footprinting, kinetic dissection of reactions and comparative sequence analysis to show that in self-splicing introns belonging to subgroup IIB, the sites that bind the 5' and 3' exons are connected to one another by tertiary interactions. This unanticipated arrangement, which contrasts with the direct covalent linkage that prevails in the other major subdivision of group II (subgroup IIA), results in a unique three-dimensional architecture for the complex between the exons, their binding sites and intron domain V. A key feature of the modeled complex is the presence of several close contacts between domain V and one of the intron-exon pairings. These contacts, whose existence is supported by hydroxyl radical footprinting, provide a structural framework for the known role of domain V in catalysis and its recently demonstrated involvement in binding of the 5' exon. A three-dimensional perspective on exon binding by a group II self-splicing intron.,Costa M, Michel F, Westhof E EMBO J. 2000 Sep 15;19(18):5007-18. PMID:10990464[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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