1fw6: Difference between revisions

Revision as of 16:50, 2 May 2008

CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX

OverviewOverview

The MutS protein initiates DNA mismatch repair by recognizing mispaired and unpaired bases embedded in duplex DNA and activating endo- and exonucleases to remove the mismatch. Members of the MutS family also possess a conserved ATPase activity that belongs to the ATP binding cassette (ABC) superfamily. Here we report the crystal structure of a ternary complex of MutS-DNA-ADP and assays of initiation of mismatch repair in conjunction with perturbation of the composite ATPase active site by mutagenesis. These studies indicate that MutS has to bind both ATP and the mismatch DNA simultaneously in order to activate the other mismatch repair proteins. We propose that the MutS ATPase activity plays a proofreading role in DNA mismatch repair, verification of mismatch recognition, and authorization of repair.

About this StructureAbout this Structure

1FW6 is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.

ReferenceReference

Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair., Junop MS, Obmolova G, Rausch K, Hsieh P, Yang W, Mol Cell. 2001 Jan;7(1):1-12. PMID:11172706 Page seeded by OCA on Fri May 2 16:50:08 2008

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OCA

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 [[Image:1fw6.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1fw6", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1fw6|  PDB=1fw6  |  SCENE=  }}
-|RELATEDENTRY=[[1ewq|1ewq]], [[1ewr|1ewr]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw6 OCA], [http://www.ebi.ac.uk/pdbsum/1fw6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fw6 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX'''
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 [[Category: Rausch, K.]]
 [[Category: Yang, W.]]
-[[Category: abc atpase]]
+[[Category: Abc atpase]]
-[[Category: kinked dna]]
+[[Category: Kinked dna]]
-[[Category: multi-domain structure]]
+[[Category: Multi-domain structure]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:50:08 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:31:26 2008''