1fqk: Difference between revisions

Revision as of 16:38, 2 May 2008

CRYSTAL STRUCTURE OF THE HETERODIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]

OverviewOverview

A multitude of heptahelical receptors use heterotrimeric G proteins to transduce signals to specific effector target molecules. The G protein transducin, Gt, couples photon-activated rhodopsin with the effector cyclic GMP phosophodiesterase (PDE) in the vertebrate phototransduction cascade. The interactions of the Gt alpha-subunit (alpha(t)) with the inhibitory PDE gamma-subunit (PDEgamma) are central to effector activation, and also enhance visual recovery in cooperation with the GTPase-activating protein regulator of G-protein signalling (RGS)-9 (refs 1-3). Here we describe the crystal structure at 2.0 A of rod transducin alpha x GDP x AlF4- in complex with the effector molecule PDEgamma and the GTPase-activating protein RGS9. In addition, we present the independently solved crystal structures of the RGS9 RGS domain both alone and in complex with alpha(t/i1) x GDP x AlF4-. These structures reveal insights into effector activation, synergistic GTPase acceleration, RGS9 specificity and RGS activity. Effector binding to a nucleotide-dependent site on alpha(t) sequesters PDEgamma residues implicated in PDE inhibition, and potentiates recruitment of RGS9 for hydrolytic transition state stabilization and concomitant signal termination.

About this StructureAbout this Structure

1FQK is a Protein complex structure of sequences from Bos taurus and rattus norvegicus and Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Structural determinants for regulation of phosphodiesterase by a G protein at 2.0 A., Slep KC, Kercher MA, He W, Cowan CW, Wensel TG, Sigler PB, Nature. 2001 Feb 22;409(6823):1071-7. PMID:11234020 Page seeded by OCA on Fri May 2 16:38:54 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1fqk.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1fqk |SIZE=350|CAPTION= <scene name='initialview01'>1fqk</scene>, resolution 2.30&Aring;
+The line below this paragraph, containing "STRUCTURE_1fqk", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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-|GENE=
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-|RELATEDENTRY=[[1fqj|1FQJ]], [[1fqi|1fqi]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fqk OCA], [http://www.ebi.ac.uk/pdbsum/1fqk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fqk RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE HETERODIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]'''
@@ Line 32: / Line 29: @@
 [[Category: Slep, K C.]]
 [[Category: Wensel, T G.]]
-[[Category: g protein]]
+[[Category: G protein]]
-[[Category: gap]]
+[[Category: Gap]]
-[[Category: phototransduction]]
+[[Category: Phototransduction]]
-[[Category: rg]]
+[[Category: Rg]]
-[[Category: rgs9]]
+[[Category: Rgs9]]
-[[Category: rod]]
+[[Category: Rod]]
-[[Category: transducin]]
+[[Category: Transducin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:38:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:28:21 2008''