2v8e: Difference between revisions
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==Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand.== | |||
<StructureSection load='2v8e' size='340' side='right' caption='[[2v8e]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='2v8e' size='340' side='right' caption='[[2v8e]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fhc|1fhc]], [[1hfh|1hfh]], [[2bzm|2bzm]], [[2g7i|2g7i]], [[2jgx|2jgx]], [[1haq|1haq]], [[1hcc|1hcc]], [[1hfi|1hfi]], [[1kov|1kov]], [[2jgw|2jgw]], [[2uwn|2uwn]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fhc|1fhc]], [[1hfh|1hfh]], [[2bzm|2bzm]], [[2g7i|2g7i]], [[2jgx|2jgx]], [[1haq|1haq]], [[1hcc|1hcc]], [[1hfi|1hfi]], [[1kov|1kov]], [[2jgw|2jgw]], [[2uwn|2uwn]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v8e OCA], [http://pdbe.org/2v8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v8e RCSB], [http://www.ebi.ac.uk/pdbsum/2v8e PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v8e OCA], [http://pdbe.org/2v8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v8e RCSB], [http://www.ebi.ac.uk/pdbsum/2v8e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v8e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v8/2v8e_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v8/2v8e_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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==See Also== | ==See Also== | ||
*[[Complement factor | *[[Complement factor|Complement factor]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Uhrin, D]] | [[Category: Uhrin, D]] | ||
[[Category: Age-related macular degeneration]] | [[Category: Age-related macular degeneration]] | ||
[[Category: Alternative splicing]] | |||
[[Category: Complement]] | [[Category: Complement]] | ||
[[Category: Complement alternate pathway]] | [[Category: Complement alternate pathway]] | ||
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[[Category: Immune system]] | [[Category: Immune system]] | ||
[[Category: Innate immunity]] | [[Category: Innate immunity]] | ||
[[Category: Polymorphism]] | |||
[[Category: Secreted]] | [[Category: Secreted]] | ||
[[Category: Sucrose octasulphate]] | [[Category: Sucrose octasulphate]] | ||
[[Category: Sushi]] | [[Category: Sushi]] | ||
Revision as of 11:49, 12 September 2018
Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand.Crystal structure of Human Complement Factor H, SCR domains 6-8 (H402 risk variant), in complex with ligand.
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedNearly 50 million people worldwide suffer from age-related macular degeneration (AMD), which causes severe loss of central vision. A single-nucleotide polymorphism in the gene for the complement regulator factor H (FH), which causes a Tyr-to-His substitution at position 402, is linked to approximately 50% of attributable risks for AMD. We present the crystal structure of the region of FH containing the polymorphic amino acid His402 in complex with an analogue of the glycosaminoglycans (GAGs) that localize the complement regulator on the cell surface. The structure demonstrates direct coordination of ligand by the disease-associated polymorphic residue, providing a molecular explanation of the genetic observation. This glycan-binding site occupies the center of an extended interaction groove on the regulator's surface, implying multivalent binding of sulfated GAGs. This finding is confirmed by structure-based site-directed mutagenesis, nuclear magnetic resonance-monitored binding experiments performed for both H402 and Y402 variants with this and another model GAG, and analysis of an extended GAG-FH complex. Structural basis for complement factor H linked age-related macular degeneration.,Prosser BE, Johnson S, Roversi P, Herbert AP, Blaum BS, Tyrrell J, Jowitt TA, Clark SJ, Tarelli E, Uhrin D, Barlow PN, Sim RB, Day AJ, Lea SM J Exp Med. 2007 Oct 1;204(10):2277-83. Epub 2007 Sep 24. PMID:17893204[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Human
- Barlow, P N
- Blaum, B S
- Clark, S J
- Day, A J
- Herbert, A P
- Johnson, S
- Jowitt, T A
- Lea, S M
- Prosser, B E
- Roversi, P
- Sim, R B
- Tarelli, E
- Tyrrell, J
- Uhrin, D
- Age-related macular degeneration
- Alternative splicing
- Complement
- Complement alternate pathway
- Disease mutation
- Factor h
- Glycoprotein
- Glycosaminoglycan
- Immune response
- Immune system
- Innate immunity
- Polymorphism
- Secreted
- Sucrose octasulphate
- Sushi