1osl: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence== | ==Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence== | ||
<StructureSection load='1osl' size='340' side='right' caption='[[1osl]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | <StructureSection load='1osl' size='340' side='right' caption='[[1osl]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| Line 5: | Line 6: | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1l1m|1l1m]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1l1m|1l1m]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LACI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LACI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1osl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osl OCA], [http://pdbe.org/1osl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1osl RCSB], [http://www.ebi.ac.uk/pdbsum/1osl PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1osl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osl OCA], [http://pdbe.org/1osl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1osl RCSB], [http://www.ebi.ac.uk/pdbsum/1osl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1osl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| Line 13: | Line 14: | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/1osl_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/1osl_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
| Line 31: | Line 32: | ||
==See Also== | ==See Also== | ||
*[[Eric Martz's Favorites|Eric Martz's Favorites]] | *[[Eric Martz's Favorites|Eric Martz's Favorites]] | ||
*[[Lac repressor morph methods|Lac repressor morph methods]] | *[[Lac repressor morph methods|Lac repressor morph methods]] | ||
== References == | == References == | ||
Revision as of 10:24, 15 February 2018
Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequenceSolution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence
Structural highlights
Function[LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedInteraction of regulatory DNA binding proteins with their target sites is usually preceded by binding to nonspecific DNA. This speeds up the search for the target site by several orders of magnitude. We report the solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA. The same set of residues can switch roles from a purely electrostatic interaction with the DNA backbone in the nonspecific complex to a highly specific binding mode with the base pairs of the cognate operator sequence. The protein-DNA interface of the nonspecific complex is flexible on biologically relevant time scales that may assist in the rapid and efficient finding of the target site. Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes.,Kalodimos CG, Biris N, Bonvin AM, Levandoski MM, Guennuegues M, Boelens R, Kaptein R Science. 2004 Jul 16;305(5682):386-9. PMID:15256668[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||