3dhd: Difference between revisions
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==Crystal structure of human NAMPT complexed with nicotinamide mononucleotide and pyrophosphate== | ==Crystal structure of human NAMPT complexed with nicotinamide mononucleotide and pyrophosphate== | ||
<StructureSection load='3dhd' size='340' side='right' caption='[[3dhd]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='3dhd' size='340' side='right' caption='[[3dhd]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NAMPT, PBEF, PBEF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NAMPT, PBEF, PBEF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamide_phosphoribosyltransferase Nicotinamide phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.12 2.4.2.12] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamide_phosphoribosyltransferase Nicotinamide phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.12 2.4.2.12] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhd OCA], [http://pdbe.org/3dhd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dhd RCSB], [http://www.ebi.ac.uk/pdbsum/3dhd PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhd OCA], [http://pdbe.org/3dhd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dhd RCSB], [http://www.ebi.ac.uk/pdbsum/3dhd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dhd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 3dhd" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3dhd" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Ho, M]] | [[Category: Ho, M]] | ||
[[Category: Schramm, V L]] | [[Category: Schramm, V L]] | ||
[[Category: Alternative splicing]] | |||
[[Category: Cytoplasm]] | |||
[[Category: Glycosyltransferase]] | [[Category: Glycosyltransferase]] | ||
[[Category: Namprtase]] | [[Category: Namprtase]] | ||
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[[Category: Nmprtase]] | [[Category: Nmprtase]] | ||
[[Category: Phosphoprotein]] | [[Category: Phosphoprotein]] | ||
[[Category: Polymorphism]] | |||
[[Category: Pyridine nucleotide biosynthesis]] | [[Category: Pyridine nucleotide biosynthesis]] | ||
[[Category: Pyrophosphate]] | [[Category: Pyrophosphate]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
[[Category: Visfatin]] | [[Category: Visfatin]] | ||
Revision as of 11:49, 25 October 2017
Crystal structure of human NAMPT complexed with nicotinamide mononucleotide and pyrophosphateCrystal structure of human NAMPT complexed with nicotinamide mononucleotide and pyrophosphate
Structural highlights
Function[NAMPT_HUMAN] Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedNicotinamide phosphoribosyltransferase (NAMPT) is highly evolved to capture nicotinamide (NAM) and replenish the nicotinamide adenine dinucleotide (NAD(+)) pool during ADP-ribosylation and transferase reactions. ATP-phosphorylation of an active-site histidine causes catalytic activation, increasing NAM affinity by 160,000. Crystal structures of NAMPT with catalytic site ligands identify the phosphorylation site, establish its role in catalysis, demonstrate unique overlapping ATP and phosphoribosyltransferase sites, and establish reaction coordinate motion. NAMPT structures with beryllium fluoride indicate a covalent H247-BeF(3)(-) as the phosphohistidine mimic. Activation of NAMPT by H247-phosphorylation causes stabilization of the enzyme-phosphoribosylpyrophosphate complex, permitting efficient capture of NAM. Reactant and product structures establish reaction coordinate motion for NAMPT to be migration of the ribosyl anomeric carbon from the pyrophosphate leaving group to the nicotinamide-N1 while the 5-phosphoryl group, the pyrophosphate moiety, and the nicotinamide ring remain fixed in the catalytic site. A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT.,Burgos ES, Ho MC, Almo SC, Schramm VL Proc Natl Acad Sci U S A. 2009 Aug 4. PMID:19666527[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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