1ffy: Difference between revisions

Revision as of 16:16, 2 May 2008

INSIGHTS INTO EDITING FROM AN ILE-TRNA SYNTHETASE STRUCTURE WITH TRNA(ILE) AND MUPIROCIN

OverviewOverview

Isoleucyl-transfer RNA (tRNA) synthetase (IleRS) joins Ile to tRNA(Ile) at its synthetic active site and hydrolyzes incorrectly acylated amino acids at its editing active site. The 2.2 angstrom resolution crystal structure of Staphylococcus aureus IleRS complexed with tRNA(Ile) and Mupirocin shows the acceptor strand of the tRNA(Ile) in the continuously stacked, A-form conformation with the 3' terminal nucleotide in the editing active site. To position the 3' terminus in the synthetic active site, the acceptor strand must adopt the hairpinned conformation seen in tRNA(Gln) complexed with its synthetase. The amino acid editing activity of the IleRS may result from the incorrect products shuttling between the synthetic and editing active sites, which is reminiscent of the editing mechanism of DNA polymerases.

About this StructureAbout this Structure

1FFY is a Single protein structure of sequence from Staphylococcus aureus. The following page contains interesting information on the relation of 1FFY with [Aminoacyl-tRNA Synthetases]. Full crystallographic information is available from OCA.

ReferenceReference

Insights into editing from an ile-tRNA synthetase structure with tRNAile and mupirocin., Silvian LF, Wang J, Steitz TA, Science. 1999 Aug 13;285(5430):1074-7. PMID:10446055 Page seeded by OCA on Fri May 2 16:16:46 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1ffy.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ffy |SIZE=350|CAPTION= <scene name='initialview01'>1ffy</scene>, resolution 2.2&Aring;
+The line below this paragraph, containing "STRUCTURE_1ffy", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MRC:MUPIROCIN'>MRC</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoleucine--tRNA_ligase Isoleucine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.5 6.1.1.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ffy|  PDB=1ffy  |  SCENE=  }}
-|RELATEDENTRY=[[1qu2|1QU2]], [[1qu3|1QU3]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ffy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ffy OCA], [http://www.ebi.ac.uk/pdbsum/1ffy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ffy RCSB]</span>
-}}
 '''INSIGHTS INTO EDITING FROM AN ILE-TRNA SYNTHETASE STRUCTURE WITH TRNA(ILE) AND MUPIROCIN'''
@@ Line 30: / Line 27: @@
 [[Category: Steitz, T A.]]
 [[Category: Wang, J.]]
-[[Category: double-sieve]]
+[[Category: Double-sieve]]
-[[Category: editing trna synthetase]]
+[[Category: Editing trna synthetase]]
-[[Category: ligase/rna]]
+[[Category: Ligase/rna]]
-[[Category: metal ion]]
+[[Category: Metal ion]]
-[[Category: mupirocin]]
+[[Category: Mupirocin]]
-[[Category: staphylococcus aureus,protein-rna complex]]
+[[Category: Staphylococcus aureus,protein-rna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:16:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:22:05 2008''