1fd2: Difference between revisions

Revision as of 16:11, 2 May 2008

SITE-DIRECTED MUTAGENESIS OF AZOTOBACTER VINELANDII FERREDOXIN I. (FE-S) CLUSTER-DRIVEN PROTEIN REARRANGEMENT

OverviewOverview

Azotobacter vinelandii ferredoxin I is a small protein that contains one [4Fe-4S] cluster and one [3Fe-4S] cluster. Recently the x-ray crystal structure has been redetermined and the fdxA gene, which encodes the protein, has been cloned and sequenced. Here we report the site-directed mutation of Cys-20, which is a ligand of the [4Fe-4S] cluster in the native protein, to alanine and the characterization of the protein product by x-ray crystallographic and spectroscopic methods. The data show that the mutant protein again contains one [4Fe-4S] cluster and one [3Fe-4S] cluster. The new [4Fe-4S] cluster obtains its fourth ligand from Cys-24, a free cysteine in the native structure. The formation of this [4Fe-4S] cluster drives rearrangement of the protein structure.

About this StructureAbout this Structure

1FD2 is a Single protein structure of sequence from Azotobacter vinelandii. Full crystallographic information is available from OCA.

ReferenceReference

Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: [Fe-S] cluster-driven protein rearrangement., Martin AE, Burgess BK, Stout CD, Cash VL, Dean DR, Jensen GM, Stephens PJ, Proc Natl Acad Sci U S A. 1990 Jan;87(2):598-602. PMID:2153958 Page seeded by OCA on Fri May 2 16:11:24 2008

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OCA

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 [[Image:1fd2.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1fd2 |SIZE=350|CAPTION= <scene name='initialview01'>1fd2</scene>, resolution 1.9&Aring;
+The line below this paragraph, containing "STRUCTURE_1fd2", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1fd2|  PDB=1fd2  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fd2 OCA], [http://www.ebi.ac.uk/pdbsum/1fd2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fd2 RCSB]</span>
-}}
 '''SITE-DIRECTED MUTAGENESIS OF AZOTOBACTER VINELANDII FERREDOXIN I. (FE-S) CLUSTER-DRIVEN PROTEIN REARRANGEMENT'''
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 [[Category: Single protein]]
 [[Category: Stout, C D.]]
-[[Category: electron transfer(iron-sulfur protein)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:11:24 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:20:32 2008''