1fcw: Difference between revisions

Revision as of 16:11, 2 May 2008

TRNA POSITIONS DURING THE ELONGATION CYCLE

OverviewOverview

Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle., Agrawal RK, Spahn CM, Penczek P, Grassucci RA, Nierhaus KH, Frank J, J Cell Biol. 2000 Aug 7;150(3):447-60. PMID:10931859 Page seeded by OCA on Fri May 2 16:11:10 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1fcw.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1fcw |SIZE=350|CAPTION= <scene name='initialview01'>1fcw</scene>, resolution 17.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1fcw", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5&#39;-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5&#39;-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5&#39;-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5&#39;-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5&#39;-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5&#39;-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=M2G:N2-DIMETHYLGUANOSINE-5&#39;-MONOPHOSPHATE'>M2G</scene>, <scene name='pdbligand=OMC:O2&#39;-METHYLYCYTIDINE-5&#39;-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2&#39;-METHYLGUANOSINE-5&#39;-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5&#39;-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=YG:WYBUTOSINE'>YG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1fcw|  PDB=1fcw  |  SCENE=  }}
-|RELATEDENTRY=[[trna09|TRNA09]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fcw OCA], [http://www.ebi.ac.uk/pdbsum/1fcw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fcw RCSB]</span>
-}}
 '''TRNA POSITIONS DURING THE ELONGATION CYCLE'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-FCW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCW OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCW OCA].
 ==Reference==
 Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle., Agrawal RK, Spahn CM, Penczek P, Grassucci RA, Nierhaus KH, Frank J, J Cell Biol. 2000 Aug 7;150(3):447-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10931859 10931859]
-[[Category: Protein complex]]
-[[Category: Saccharomyces cerevisiae]]
 [[Category: Agrawal, R K.]]
 [[Category: Frank, J.]]
@@ Line 31: / Line 26: @@
 [[Category: Penczek, P.]]
 [[Category: Spahn, C M.T.]]
-[[Category: cryo-electron microscopy]]
+[[Category: Cryo-electron microscopy]]
-[[Category: elongation cycle]]
+[[Category: Elongation cycle]]
-[[Category: protein synthesis]]
+[[Category: Protein synthesis]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
-[[Category: trna binding site]]
+[[Category: Trna binding site]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:11:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:20:29 2008''