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'''THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FROM A MONOCLONAL ANTI-ARSONATE ANTIBODY''' | '''THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FROM A MONOCLONAL ANTI-ARSONATE ANTIBODY''' | ||
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==About this Structure== | ==About this Structure== | ||
1FAI is a [[Single protein]] structure | 1FAI is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FAI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Nisonoff, A.]] | [[Category: Nisonoff, A.]] | ||
[[Category: Poljak, R J.]] | [[Category: Poljak, R J.]] | ||
[[Category: | [[Category: Immunoglobulin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:06:14 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 16:06, 2 May 2008
THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FROM A MONOCLONAL ANTI-ARSONATE ANTIBODY
OverviewOverview
The three-dimensional structure of FabR19.9 from a well-characterized anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray diffraction techniques in two crystalline forms (I and II) to a resolution of 2.8 and 2.7 A, respectively. Essentially the same tertiary and quaternary structure of the Fab is observed in the two forms. The major difference resides in the intermolecular contacts, which are interpreted to favor an irreversible transition from the metastable form I to the more stable form II. The third complementarity-determining region of the heavy chain (H3) folds back over the combining site and requires rearrangement for hapten binding. This dynamic requirement on H3 is consistent with its mobility in the structure and can explain hapten binding to an otherwise inaccessible antibody combining site.
About this StructureAbout this Structure
1FAI is a Single protein structure. Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional structure of two crystal forms of FabR19.9 from a monoclonal anti-arsonate antibody., Lascombe MB, Alzari PM, Poljak RJ, Nisonoff A, Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9429-33. PMID:1409652 Page seeded by OCA on Fri May 2 16:06:14 2008