1f9k: Difference between revisions

Revision as of 16:04, 2 May 2008

WINGED BEAN ACIDIC LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE

OverviewOverview

Structures of two crystal forms of the dimeric acidic winged bean agglutinin (WBAII) complexed with methyl-alpha-D-galactose have been determined at 3.0 A and 3.3 A resolution. The subunit structure and dimerisation of the lectin are similar to those of the basic lectin from winged bean (WBAI) and the lectin from Erythrina corallodendron (EcorL). The conformation of a loop and its orientation with respect to the rest of the molecule in WBAII are, however, different from those in all the other legume lectins of known structure. This difference appears to have been caused by the formation of two strategically placed salt bridges in the former. Modelling based on the crystal structures provides a rationale for the specificity of the lectin, which is very different from that of WBAI, for the H-antigenic determinant responsible for O blood group reactivity. It also leads to a qualitative explanation for the thermodynamic data on sugar-binding to the lectin, with special emphasis on the role of a tyrosyl residue in the variable loop in the sugar-binding region in generating the carbohydrate specificity of WBAII.

About this StructureAbout this Structure

1F9K is a Single protein structure of sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA.

ReferenceReference

Carbohydrate specificity and salt-bridge mediated conformational change in acidic winged bean agglutinin., Manoj N, Srinivas VR, Surolia A, Vijayan M, Suguna K, J Mol Biol. 2000 Oct 6;302(5):1129-37. PMID:11183779 Page seeded by OCA on Fri May 2 16:04:17 2008

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OCA

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 [[Image:1f9k.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1f9k", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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-|GENE=
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-|RELATEDENTRY=[[1fay|1FAY]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f9k OCA], [http://www.ebi.ac.uk/pdbsum/1f9k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f9k RCSB]</span>
-}}
 '''WINGED BEAN ACIDIC LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE'''
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 [[Category: Surolia, A.]]
 [[Category: Vijayan, M.]]
-[[Category: agglutinin]]
+[[Category: Agglutinin]]
-[[Category: glycosylated protein]]
+[[Category: Glycosylated protein]]
-[[Category: h-antigenic specificity]]
+[[Category: H-antigenic specificity]]
-[[Category: legume lectin]]
+[[Category: Legume lectin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:04:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:18:35 2008''