1f8g: Difference between revisions

Revision as of 16:01, 2 May 2008

THE X-RAY STRUCTURE OF NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM COMPLEXED WITH NAD+

OverviewOverview

BACKGROUND: Membrane-bound ion pumps are involved in metabolic regulation, osmoregulation, cell signalling, nerve transmission and energy transduction. How the ion electrochemical gradient interacts with the scalar chemistry and how the catalytic machinery is gated to ensure high coupling efficiency are fundamental to the mechanism of action of such pumps. Transhydrogenase is a conformationally coupled proton pump linking a proton gradient to the redox reaction between NAD(H) and NADP(H). The enzyme has three components; dI binds NAD(H), dII spans the membrane and dIII binds NADP(H). RESULTS: The first crystal structure of a transhydrogenase dI component (from Rhodospirillum rubrum) has been determined at 2.0 A resolution. The monomer comprises two domains. Both are involved in dimer formation, and one has a Rossmann fold that binds NAD+ in a novel mode. The two domains can adopt different conformations. In the most closed conformation, the nicotinamide ring is expelled from the cleft between the two domains and is exposed on the outside of the protein. In this conformation it is possible to dock the structure of dI/NAD+ with that of a dIII/NADP+ complex to provide the first insights into the molecular basis of the hydride-transfer step. CONCLUSIONS: Analysis of the model of the dI/dIII complex identifies residues potentially involved in dI/dIII interaction and shows how domain motion in dI results in a shift in position of the nicotinamide ring of NAD+. We propose that this movement is responsible for switching between the forbidden and allowed states for hydride transfer during proton pumping.

About this StructureAbout this Structure

1F8G is a Single protein structure of sequence from Rhodospirillum rubrum. Full crystallographic information is available from OCA.

ReferenceReference

Protein-protein recognition, hydride transfer and proton pumping in the transhydrogenase complex., Buckley PA, Baz Jackson J, Schneider T, White SA, Rice DW, Baker PJ, Structure. 2000 Aug 15;8(8):809-15. PMID:10997900 Page seeded by OCA on Fri May 2 16:01:41 2008

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OCA

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 [[Image:1f8g.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1f8g |SIZE=350|CAPTION= <scene name='initialview01'>1f8g</scene>, resolution 2.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1f8g", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(P)(+)_transhydrogenase_(B-specific) NAD(P)(+) transhydrogenase (B-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.1.1 1.6.1.1] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f8g OCA], [http://www.ebi.ac.uk/pdbsum/1f8g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f8g RCSB]</span>
-}}
 '''THE X-RAY STRUCTURE OF NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM COMPLEXED WITH NAD+'''
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 ==Reference==
 Protein-protein recognition, hydride transfer and proton pumping in the transhydrogenase complex., Buckley PA, Baz Jackson J, Schneider T, White SA, Rice DW, Baker PJ, Structure. 2000 Aug 15;8(8):809-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10997900 10997900]
-[[Category: NAD(P)(+) transhydrogenase (B-specific)]]
 [[Category: Rhodospirillum rubrum]]
 [[Category: Single protein]]
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 [[Category: Schneider, T.]]
 [[Category: White, S A.]]
-[[Category: nucleotide fold]]
+[[Category: Nucleotide fold]]
-[[Category: proton pump transhydrogenase]]
+[[Category: Proton pump transhydrogenase]]
-[[Category: rossmann fold]]
+[[Category: Rossmann fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:01:41 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:18:01 2008''