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==Crystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNA== | ==Crystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNA== | ||
<StructureSection load='3fbd' size='340' side='right' caption='[[3fbd]], [[Resolution|resolution]] 2.90Å' scene=''> | <StructureSection load='3fbd' size='340' side='right' caption='[[3fbd]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ivh|2ivh]], [[1zns|1zns]], [[1pt3|1pt3]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ivh|2ivh]], [[1zns|1zns]], [[1pt3|1pt3]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cea, colE7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cea, colE7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fbd OCA], [http://pdbe.org/3fbd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fbd RCSB], [http://www.ebi.ac.uk/pdbsum/3fbd PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fbd OCA], [http://pdbe.org/3fbd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fbd RCSB], [http://www.ebi.ac.uk/pdbsum/3fbd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fbd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/3fbd_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/3fbd_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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[[Category: Metal-binding]] | [[Category: Metal-binding]] | ||
[[Category: Nuclease]] | [[Category: Nuclease]] | ||
[[Category: Plasmid]] | |||
[[Category: Protein engineering]] | [[Category: Protein engineering]] | ||
[[Category: Protein-nucleic acid interaction]] | [[Category: Protein-nucleic acid interaction]] | ||
[[Category: Zinc]] | |||
Revision as of 10:36, 5 December 2018
Crystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNACrystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNA
Structural highlights
Function[CEA7_ECOLX] This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. See Also |
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