1eoe: Difference between revisions

Revision as of 15:20, 2 May 2008

CRYSTAL STRUCTURE OF THE V135R MUTANT OF A SHAKER T1 DOMAIN

OverviewOverview

The T1 domain, a highly conserved cytoplasmic portion at the N-terminus of the voltage-dependent K+ channel (Kv) alpha-subunit, is responsible for driving and regulating the tetramerization of the alpha-subunits. Here we report the identification of a set of mutations in the T1 domain that alter the gating properties of the Kv channel. Two mutants produce a leftward shift in the activation curve and slow the channel closing rate while a third mutation produces a rightward shift in the activation curve and speeds the channel closing rate. We have determined the crystal structures of T1 domains containing these mutations. Both of the leftward shifting mutants produce similar conformational changes in the putative membrane facing surface of the T1 domain. These results suggest that the structure of the T1 domain in this region is tightly coupled to the channel's gating states.

About this StructureAbout this Structure

1EOE is a Single protein structure of sequence from Aplysia californica. Full crystallographic information is available from OCA.

ReferenceReference

Voltage dependent activation of potassium channels is coupled to T1 domain structure., Cushman SJ, Nanao MH, Jahng AW, DeRubeis D, Choe S, Pfaffinger PJ, Nat Struct Biol. 2000 May;7(5):403-7. PMID:10802739 Page seeded by OCA on Fri May 2 15:20:43 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1eoe.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1eoe |SIZE=350|CAPTION= <scene name='initialview01'>1eoe</scene>, resolution 1.704&Aring;
+The line below this paragraph, containing "STRUCTURE_1eoe", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1eoe|  PDB=1eoe  |  SCENE=  }}
-|RELATEDENTRY=[[1eod|1EOD]], [[1eof|1EOF]], [[1t1d|1T1D]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoe OCA], [http://www.ebi.ac.uk/pdbsum/1eoe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eoe RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE V135R MUTANT OF A SHAKER T1 DOMAIN'''
@@ Line 31: / Line 28: @@
 [[Category: Nanao, M H.]]
 [[Category: Pfaffinger, P J.]]
-[[Category: aplysia kv1 1]]
+[[Category: Aplysia kv1 1]]
-[[Category: potassium channel]]
+[[Category: Potassium channel]]
-[[Category: proton transport]]
+[[Category: Proton transport]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:20:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:06:41 2008''