1ejj: Difference between revisions

Revision as of 15:10, 2 May 2008

CRYSTAL STRUCTURAL ANALYSIS OF PHOSPHOGLYCERATE MUTASE COCRYSTALLIZED WITH 3-PHOSPHOGLYCERATE

OverviewOverview

Bacillus stearothermophilus phosphoglycerate mutase (PGM), which interconverts 2- and 3-phosphoglyceric acid (PGA), does not require 2,3-diphosphoglyceric acid for activity. However, this enzyme does have an absolute and specific requirement for Mn(2+) ions for catalysis. Here we report the crystal structure of this enzyme complexed with 3PGA and manganese ions to 1.9 A resolution; this is the first crystal structure of a diphosphoglycerate-independent PGM to be determined. This information, plus the location of the two bound Mn(2+) ions and the 3PGA have allowed formulation of a possible catalytic mechanism for this PGM. In this mechanism Mn(2+) ions facilitate the transfer of the substrate's phosphate group to Ser62 to form a phosphoserine intermediate. In the subsequent phosphotransferase part of the reaction, the phosphate group is transferred from Ser62 to the O2 or O3 positions of the reoriented glycerate to yield the PGA product. Site-directed mutagenesis studies were used to confirm our mechanism and the involvement of specific enzyme residues in Mn(2+) binding and catalysis.

About this StructureAbout this Structure

1EJJ is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus., Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G, EMBO J. 2000 Apr 3;19(7):1419-31. PMID:10747010 Page seeded by OCA on Fri May 2 15:10:46 2008

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OCA

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 [[Image:1ejj.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ejj |SIZE=350|CAPTION= <scene name='initialview01'>1ejj</scene>, resolution 1.90&Aring;
+The line below this paragraph, containing "STRUCTURE_1ejj", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_mutase Phosphoglycerate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.1 5.4.2.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ejj|  PDB=1ejj  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ejj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ejj OCA], [http://www.ebi.ac.uk/pdbsum/1ejj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ejj RCSB]</span>
-}}
 '''CRYSTAL STRUCTURAL ANALYSIS OF PHOSPHOGLYCERATE MUTASE COCRYSTALLIZED WITH 3-PHOSPHOGLYCERATE'''
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 [[Category: Krishnasamy, G.]]
 [[Category: Setlow, P.]]
-[[Category: alpha/beta-type structure]]
+[[Category: Alpha/beta-type structure]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:10:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:03:50 2008''