3jz0: Difference between revisions
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==LinB complexed with clindamycin and AMPCPP== | ==LinB complexed with clindamycin and AMPCPP== | ||
<StructureSection load='3jz0' size='340' side='right' caption='[[3jz0]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='3jz0' size='340' side='right' caption='[[3jz0]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3jyy|3jyy]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3jyy|3jyy]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">linB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">linB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jz0 OCA], [http://pdbe.org/3jz0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jz0 RCSB], [http://www.ebi.ac.uk/pdbsum/3jz0 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jz0 OCA], [http://pdbe.org/3jz0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jz0 RCSB], [http://www.ebi.ac.uk/pdbsum/3jz0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jz0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
Revision as of 11:22, 1 November 2017
LinB complexed with clindamycin and AMPCPPLinB complexed with clindamycin and AMPCPP
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedLincosamides make up an important class of antibiotics used against a wide range of pathogens, including methicillin-resistant Staphylococcus aureus. Predictably, lincosamide-resistant microorganisms have emerged with antibiotic modification as one of their major resistance strategies. Inactivating enzymes LinB/A catalyze adenylylation of the drug; however, little is known about their mechanistic and structural properties. We determined two X-ray structures of LinB: ternary substrate- and binary product-bound complexes. Structural and kinetic characterization of LinB, mutagenesis, solvent isotope effect, and product inhibition studies are consistent with a mechanism involving direct in-line nucleotidyl transfer. The characterization of LinB enabled its classification as a member of a nucleotidyltransferase superfamily, along with nucleotide polymerases and aminoglycoside nucleotidyltransferases, and this relationship offers further support for the LinB mechanism. The LinB structure provides an evolutionary link to ancient nucleotide polymerases and suggests that, like protein kinases and acetyltransferases, these are proto-resistance elements from which drug resistance can evolve. Structure and mechanism of the lincosamide antibiotic adenylyltransferase LinB.,Morar M, Bhullar K, Hughes DW, Junop M, Wright GD Structure. 2009 Dec 9;17(12):1649-59. PMID:20004168[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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