1eej: Difference between revisions

Revision as of 14:59, 2 May 2008

CRYSTAL STRUCTURE OF THE PROTEIN DISULFIDE BOND ISOMERASE, DSBC, FROM ESCHERICHIA COLI

OverviewOverview

DsbC is one of five Escherichia coli proteins required for disulfide bond formation and is thought to function as a disulfide bond isomerase during oxidative protein folding in the periplasm. DsbC is a 2 x 23 kDa homodimer and has both protein disulfide isomerase and chaperone activity. We report the 1.9 A resolution crystal structure of oxidized DsbC where both Cys-X-X-Cys active sites form disulfide bonds. The molecule consists of separate thioredoxin-like domains joined via hinged linker helices to an N-terminal dimerization domain. The hinges allow relative movement of the active sites, and a broad uncharged cleft between them may be involved in peptide binding and DsbC foldase activities.

About this StructureAbout this Structure

1EEJ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli., McCarthy AA, Haebel PW, Torronen A, Rybin V, Baker EN, Metcalf P, Nat Struct Biol. 2000 Mar;7(3):196-9. PMID:10700276 Page seeded by OCA on Fri May 2 14:59:54 2008

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OCA

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 [[Image:1eej.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1eej |SIZE=350|CAPTION= <scene name='initialview01'>1eej</scene>, resolution 1.90&Aring;
+The line below this paragraph, containing "STRUCTURE_1eej", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1eej|  PDB=1eej  |  SCENE=  }}
-|RELATEDENTRY=[[2trx|2TRX]], [[1dsb|1DSB]], [[1a2j|1A2J]], [[1a2m|1A2M]], [[1a2l|1A2L]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eej OCA], [http://www.ebi.ac.uk/pdbsum/1eej PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eej RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE PROTEIN DISULFIDE BOND ISOMERASE, DSBC, FROM ESCHERICHIA COLI'''
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 [[Category: Rybin, V.]]
 [[Category: Torronen, A.]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: protein disulfide isomerase]]
+[[Category: Protein disulfide isomerase]]
-[[Category: protein folding]]
+[[Category: Protein folding]]
-[[Category: redox protein]]
+[[Category: Redox protein]]
-[[Category: redox-active center]]
+[[Category: Redox-active center]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:59:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:01:01 2008''