3f2c: Difference between revisions
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==DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP and Mn== | ==DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP and Mn== | ||
<StructureSection load='3f2c' size='340' side='right' caption='[[3f2c]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='3f2c' size='340' side='right' caption='[[3f2c]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3f2b|3f2b]], [[3f2d|3f2d]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3f2b|3f2b]], [[3f2d|3f2d]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f2c OCA], [http://pdbe.org/3f2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f2c RCSB], [http://www.ebi.ac.uk/pdbsum/3f2c PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f2c OCA], [http://pdbe.org/3f2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f2c RCSB], [http://www.ebi.ac.uk/pdbsum/3f2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f2c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 3f2c" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3f2c" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 08:09, 16 November 2017
DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP and MnDNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP and Mn
Structural highlights
Function[Q5L0J3_GEOKA] Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity (By similarity).[HAMAP-Rule:MF_00356][SAAS:SAAS029460_004_008054] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedPolC is the polymerase responsible for genome duplication in many Gram-positive bacteria and represents an attractive target for antibacterial development. We have determined the 2.4-A resolution crystal structure of Geobacillus kaustophilus PolC in a ternary complex with DNA and dGTP. The structure reveals nascent base pair interactions that lead to highly accurate nucleotide incorporation. A unique beta-strand motif in the PolC thumb domain contacts the minor groove, allowing replication errors to be sensed up to 8 nt upstream of the active site. PolC exhibits the potential for large-scale conformational flexibility, which could encompass the catalytic residues. The structure suggests a mechanism by which the active site can communicate with the rest of the replisome to trigger proofreading after nucleotide misincorporation, leading to an integrated model for controlling the dynamic switch between replicative and repair polymerases. This ternary complex of a cellular replicative polymerase affords insights into polymerase fidelity, evolution, and structural diversity. Structure of PolC reveals unique DNA binding and fidelity determinants.,Evans RJ, Davies DR, Bullard JM, Christensen J, Green LS, Guiles JW, Pata JD, Ribble WK, Janjic N, Jarvis TC Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20695-700. Epub 2008 Dec 23. PMID:19106298[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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