1ed6: Difference between revisions

Revision as of 14:57, 2 May 2008

BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN COMPLEXED WITH L-NIO (H4B FREE)

OverviewOverview

The crystal structure of the endothelial nitric oxide synthase (NOS) heme domain complexed with NO reveals close hydrogen bonding interactions between NO and the terminal guanidino nitrogen of the substrate, L-arginine. Dioxygen is expected to bind in a similar mode which will facilitate proton abstraction from L-Arg to dioxygen, a required step for O-O bond cleavage. Structures of mechanism-based NOS inhibitors, N(5)-(1-iminoethyl)-L-ornithine and N-(3-(aminomethyl)benzyl)acetamidine, provide clues on how this class of compounds operate as suicide substrate inhibitors leading to heme oxidation.

About this StructureAbout this Structure

1ED6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Crystallographic studies on endothelial nitric oxide synthase complexed with nitric oxide and mechanism-based inhibitors., Li H, Raman CS, Martasek P, Masters BS, Poulos TL, Biochemistry. 2001 May 8;40(18):5399-406. PMID:11331003 Page seeded by OCA on Fri May 2 14:57:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1ed6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ed6 |SIZE=350|CAPTION= <scene name='initialview01'>1ed6</scene>, resolution 2.05&Aring;
+The line below this paragraph, containing "STRUCTURE_1ed6", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CAD:CACODYLIC+ACID'>CAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ILO:N5-IMINOETHYL-L-ORNITHINE'>ILO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ed6|  PDB=1ed6  |  SCENE=  }}
-|RELATEDENTRY=[[1nse|1NSE]], [[1ed4|1ED4]], [[1ed5|1ED5]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ed6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ed6 OCA], [http://www.ebi.ac.uk/pdbsum/1ed6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ed6 RCSB]</span>
-}}
 '''BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN COMPLEXED WITH L-NIO (H4B FREE)'''
@@ Line 32: / Line 29: @@
 [[Category: Raman, C S.]]
 [[Category: Southan, G J.]]
-[[Category: alpha-beta fold]]
+[[Category: Alpha-beta fold]]
-[[Category: heme protein]]
+[[Category: Heme protein]]
-[[Category: nitric oxide synthase]]
+[[Category: Nitric oxide synthase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:57:21 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:00:14 2008''