1e9z: Difference between revisions

Revision as of 14:51, 2 May 2008

CRYSTAL STRUCTURE OF HELICOBACTER PYLORI UREASE

OverviewOverview

Helicobacter pylori, an etiologic agent in a variety of gastroduodenal diseases, produces a large amount of urease, which is believed to neutralize gastric acid by producing ammonia for the survival of the bacteria. Up to 30% of the enzyme associates with the surface of intact cells upon lysis of neighboring bacteria. The role of the enzyme at the extracellular location has been a subject of controversy because the purified enzyme is irreversibly inactivated below pH 5. We have determined the crystal structure of H. pylori urease, which has a 1.1 MDa spherical assembly of 12 catalytic units with an outer diameter of approximately 160 A. Under physiologically relevant conditions, the activity of the enzyme remains unaffected down to pH 3. Activity assays under different conditions indicated that the cluster of the 12 active sites on the supramolecular assembly may be critical for the survival of the enzyme at low pH. The structure provides a novel example of a molecular assembly adapted for acid resistance that, together with the low Km value of the enzyme, is likely to enable the organism to inhabit the hostile niche.

About this StructureAbout this Structure

1E9Z is a Protein complex structure of sequences from Helicobacter pylori. Full crystallographic information is available from OCA.

ReferenceReference

Supramolecular assembly and acid resistance of Helicobacter pylori urease., Ha NC, Oh ST, Sung JY, Cha KA, Lee MH, Oh BH, Nat Struct Biol. 2001 Jun;8(6):505-9. PMID:11373617 Page seeded by OCA on Fri May 2 14:51:11 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1e9z.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1e9z |SIZE=350|CAPTION= <scene name='initialview01'>1e9z</scene>, resolution 3.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1e9z", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=NI1:Ni+Binding+Site+For+Residue+B2002'>NI1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1e9z|  PDB=1e9z  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e9z OCA], [http://www.ebi.ac.uk/pdbsum/1e9z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e9z RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF HELICOBACTER PYLORI UREASE'''
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 [[Category: Oh, B H.]]
 [[Category: Oh, S T.]]
-[[Category: dodecamer]]
+[[Category: Dodecamer]]
-[[Category: helicobacter]]
+[[Category: Helicobacter]]
-[[Category: urease]]
+[[Category: Urease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:51:11 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:58:19 2008''