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==Proteinase K by Classical hanging drop method after the first step of high X-Ray dose on ESRF ID23-1 beamline== | ==Proteinase K by Classical hanging drop method after the first step of high X-Ray dose on ESRF ID23-1 beamline== | ||
<StructureSection load='3de4' size='340' side='right' caption='[[3de4]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='3de4' size='340' side='right' caption='[[3de4]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3d9q|3d9q]], [[3ddz|3ddz]], [[3de0|3de0]], [[3de1|3de1]], [[3de2|3de2]], [[3de3|3de3]], [[3de5|3de5]], [[3de6|3de6]], [[3de7|3de7]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3d9q|3d9q]], [[3ddz|3ddz]], [[3de0|3de0]], [[3de1|3de1]], [[3de2|3de2]], [[3de3|3de3]], [[3de5|3de5]], [[3de6|3de6]], [[3de7|3de7]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3de4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3de4 OCA], [http://pdbe.org/3de4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3de4 RCSB], [http://www.ebi.ac.uk/pdbsum/3de4 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3de4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3de4 OCA], [http://pdbe.org/3de4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3de4 RCSB], [http://www.ebi.ac.uk/pdbsum/3de4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3de4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/de/3de4_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/de/3de4_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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[[Category: Tripathi, S K]] | [[Category: Tripathi, S K]] | ||
[[Category: Alpha beta protein]] | [[Category: Alpha beta protein]] | ||
[[Category: Calcium]] | |||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Metal-binding]] | [[Category: Metal-binding]] | ||
Revision as of 12:52, 14 November 2018
Proteinase K by Classical hanging drop method after the first step of high X-Ray dose on ESRF ID23-1 beamlineProteinase K by Classical hanging drop method after the first step of high X-Ray dose on ESRF ID23-1 beamline
Structural highlights
Function[PRTK_TRIAL] Hydrolyzes keratin at aromatic and hydrophobic residues. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedA detailed analysis of structural and intensity changes induced by X-ray radiation is presented for two types of proteinase K crystals: crystal grown by classical hanging drop method and those grown by Langmuir-Blodgett (LB) nanotemplate. The comparison of various parameters (e.g. intensity per sigma ratio, unit-cell volume, number of unique reflections, B-factors) and electron density maps as a function of radiation dose, demonstrates that crystals, grown by the LB nanotemplate method, appear to be more resistant against radiation damage than crystals grown by the classical hanging drop method. Radiation stability of proteinase K crystals grown by LB nanotemplate method.,Pechkova E, Tripathi S, Ravelli RB, McSweeney S, Nicolini C J Struct Biol. 2009 Dec;168(3):409-18. Epub 2009 Aug 15. PMID:19686853[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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