1e6e: Difference between revisions

Revision as of 14:43, 2 May 2008

ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS

OverviewOverview

The steroid hydroxylating system of adrenal cortex mitochondria consists of the membrane-attached NADPH-dependent adrenodoxin reductase (AR), the soluble one-electron transport protein adrenodoxin (Adx), and a membrane-integrated cytochrome P450 of the CYP11 family. In the 2.3-A resolution crystal structure of the Adx.AR complex, 580 A(2) of partly polar surface are buried. Main interaction sites are centered around Asp(79), Asp(76), Asp(72), and Asp(39) of Adx and around Arg(211), Arg(240), Arg(244), and Lys(27) of AR, respectively. In particular, the region around Asp(39) defines a new protein interaction site for Adx, similar to those found in plant and bacterial ferredoxins. Additional contacts involve the electron transfer region between the redox centers of AR and Adx and C-terminal residues of Adx. The Adx residues Asp(113) to Arg(115) adopt 3(10)-helical conformation and engage in loose intermolecular contacts within a deep cleft of AR. Complex formation is accompanied by a slight domain rearrangement in AR. The [2Fe-2S] cluster of Adx and the isoalloxazine rings of FAD of AR are 10 A apart suggesting a possible electron transfer route between these redox centers. The AR.Adx complex represents the first structure of a biologically relevant complex between a ferredoxin and its reductase.

About this StructureAbout this Structure

1E6E is a Protein complex structure of sequences from Bos taurus. The following page contains interesting information on the relation of 1E6E with [Cytochrome p450]. Full crystallographic information is available from OCA.

ReferenceReference

Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis., Muller JJ, Lapko A, Bourenkov G, Ruckpaul K, Heinemann U, J Biol Chem. 2001 Jan 26;276(4):2786-9. Epub 2000 Oct 25. PMID:11053423 Page seeded by OCA on Fri May 2 14:43:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1e6e.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1e6e |SIZE=350|CAPTION= <scene name='initialview01'>1e6e</scene>, resolution 2.3&Aring;
+The line below this paragraph, containing "STRUCTURE_1e6e", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Fad+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC5:Fes+Binding+Site+For+Chain+B'>AC5</scene>, <scene name='pdbsite=AC6:Fad+Binding+Site+For+Chain+C'>AC6</scene> and <scene name='pdbsite=AC9:Fes+Binding+Site+For+Chain+D'>AC9</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1e6e|  PDB=1e6e  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e6e OCA], [http://www.ebi.ac.uk/pdbsum/1e6e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e6e RCSB]</span>
-}}
 '''ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS'''
@@ Line 25: / Line 22: @@
 [[Category: Bos taurus]]
 [[Category: Cytochrome p450]]
-[[Category: Ferredoxin--NADP(+) reductase]]
 [[Category: Protein complex]]
 [[Category: Bourenkov, G.]]
@@ Line 32: / Line 28: @@
 [[Category: Mueller, J J.]]
 [[Category: Ruckpaul, K.]]
-[[Category: [2fe-2s]ferredoxin]]
+[[Category: Adrenodoxin]]
-[[Category: adrenodoxin]]
+[[Category: Complex]]
-[[Category: complex]]
+[[Category: Electron transferase]]
-[[Category: electron transferase]]
+[[Category: Electron transport]]
-[[Category: electron transport]]
+[[Category: Flavoenzyme]]
-[[Category: flavoenzyme]]
+[[Category: Oxidoreductase]]
-[[Category: oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:43:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:56:11 2008''