1e4p: Difference between revisions

Revision as of 14:39, 2 May 2008

STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE

OverviewOverview

The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:11142381 Page seeded by OCA on Fri May 2 14:39:32 2008

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OCA

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 [[Image:1e4p.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1e4p", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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-|GENE=
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e4p OCA], [http://www.ebi.ac.uk/pdbsum/1e4p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e4p RCSB]</span>
-}}
 '''STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE'''
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 ==About this Structure==
-E4P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA].
 ==Reference==
 Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11142381 11142381]
-[[Category: Protein complex]]
 [[Category: Heus, H A.]]
 [[Category: Hilbers, C W.]]
 [[Category: Michiels, P J.A.]]
 [[Category: Schouten, C H.J.]]
-[[Category: rna ribozyme]]
+[[Category: Rna ribozyme]]
-[[Category: substrate hairpin]]
+[[Category: Substrate hairpin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:39:32 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:55:06 2008''