1tk7: Difference between revisions
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==NMR structure of WW domains (WW3-4) from Suppressor of Deltex== | ==NMR structure of WW domains (WW3-4) from Suppressor of Deltex== | ||
<StructureSection load='1tk7' size='340' side='right' caption='[[1tk7]], [[NMR_Ensembles_of_Models | 7 NMR models]]' scene=''> | <StructureSection load='1tk7' size='340' side='right' caption='[[1tk7]], [[NMR_Ensembles_of_Models | 7 NMR models]]' scene=''> | ||
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<table><tr><td colspan='2'>[[1tk7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TK7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1tk7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TK7 FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Su(dx) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Su(dx) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tk7 OCA], [http://pdbe.org/1tk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/1tk7 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tk7 OCA], [http://pdbe.org/1tk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/1tk7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tk7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tk7_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tk7_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
Revision as of 11:47, 21 March 2018
NMR structure of WW domains (WW3-4) from Suppressor of DeltexNMR structure of WW domains (WW3-4) from Suppressor of Deltex
Structural highlights
Function[SUDX_DROME] E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Notch/N signaling pathway, probably by promoting Notch ubiquitination, endocytosis and degradation. Involved in wing growth and leg joint formation.[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedWW domains mediate protein recognition, usually though binding to proline-rich sequences. In many proteins, WW domains occur in tandem arrays. Whether or how individual domains within such arrays cooperate to recognize biological partners is, as yet, poorly characterized. An important question is whether functional diversity of different WW domain proteins is reflected in the structural organization and ligand interaction mechanisms of their multiple domains. We have determined the solution structure and dynamics of a pair of WW domains (WW3-4) from a Drosophila Nedd4 family protein called Suppressor of deltex (Su(dx)), a regulator of Notch receptor signaling. We find that the binding of a type 1 PPPY ligand to WW3 stabilizes the structure with effects propagating to the WW4 domain, a domain that is not active for ligand binding. Both WW domains adopt the characteristic triple-stranded beta-sheet structure, and significantly, this is the first example of a WW domain structure to include a domain (WW4) lacking the second conserved Trp (replaced by Phe). The domains are connected by a flexible linker, which allows a hinge-like motion of domains that may be important for the recognition of functionally relevant targets. Our results contrast markedly with those of the only previously determined three-dimensional structure of tandem WW domains, that of the rigidly oriented WW domain pair from the RNA-splicing factor Prp40. Our data illustrate that arrays of WW domains can exhibit a variety of higher order structures and ligand interaction mechanisms. The structure and dynamics of tandem WW domains in a negative regulator of notch signaling, Suppressor of deltex.,Fedoroff OY, Townson SA, Golovanov AP, Baron M, Avis JM J Biol Chem. 2004 Aug 13;279(33):34991-5000. Epub 2004 Jun 1. PMID:15173166[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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