1nhc: Difference between revisions
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==Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger== | ==Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger== | ||
<StructureSection load='1nhc' size='340' side='right' caption='[[1nhc]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='1nhc' size='340' side='right' caption='[[1nhc]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1czf|1czf]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1czf|1czf]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polygalacturonase Polygalacturonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.15 3.2.1.15] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polygalacturonase Polygalacturonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.15 3.2.1.15] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nhc OCA], [http://pdbe.org/1nhc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nhc RCSB], [http://www.ebi.ac.uk/pdbsum/1nhc PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nhc OCA], [http://pdbe.org/1nhc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nhc RCSB], [http://www.ebi.ac.uk/pdbsum/1nhc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nhc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nh/1nhc_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nh/1nhc_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1nhc" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1nhc" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Polygalacturonase|Polygalacturonase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 10:09, 7 February 2018
Structural insights into the processivity of endopolygalacturonase I from Aspergillus nigerStructural insights into the processivity of endopolygalacturonase I from Aspergillus niger
Structural highlights
Function[PGLR1_ASPNG] Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedEndopolygalacturonase I is a processive enzyme, while the 60% sequence identical endopolygalacturonase II is not. The 1.70 A resolution crystal structure of endopolygalacturonase I reveals a narrowed substrate binding cleft. In addition, Arg96, a residue in this cleft previously shown to be critical for processivity, interacts with the substrate mimics glycerol and sulfate in several well-defined conformations in the six molecules in the asymmetric unit. From this we conclude that both Arg96 and the narrowed substrate binding cleft contribute to retaining the substrate while it moves through the active site after a cleavage event has occurred. Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger.,van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:14623112[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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