1dw5: Difference between revisions

Revision as of 14:21, 2 May 2008

NMR STRUCTURE OF OMEGA-CONOTOXIN MVIIA: NO CONSTRAINTS ON DISULPHIDE BRIDGES

OverviewOverview

omega-Conotoxin MVIIA is a 25-residue, disulfide-bridged polypeptide from the venom of the sea snail Conus magus that binds to neuronal N-type calcium channels. It forms a compact folded structure, presenting a loop between Cys8 and Cys15 that contains a set of residues critical for its binding. The loop does not have a unique defined structure, nor is it intrinsically flexible. Broadening of a subset of resonances in the NMR spectrum at low temperature, anomalous temperature dependence of the chemical shifts of some resonances, and exchange contributions to J(0) from (13)C relaxation measurements reveal that conformational exchange affects the residues in this loop. The effects of this exchange on the calculated structure of omega-conotoxin MVIIA are discussed. The exchange appears to be associated with a change in the conformation of the disulfide bridge Cys8-Cys20. The implications for the use of the omega-conotoxins as a scaffold for carrying other functions is discussed.

About this StructureAbout this Structure

1DW5 is a Single protein structure. Full crystallographic information is available from OCA.

ReferenceReference

Structural and dynamic characterization of omega-conotoxin MVIIA: the binding loop exhibits slow conformational exchange., Atkinson RA, Kieffer B, Dejaegere A, Sirockin F, Lefevre JF, Biochemistry. 2000 Apr 11;39(14):3908-19. PMID:10747778 Page seeded by OCA on Fri May 2 14:21:18 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1dw5.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1dw5 |SIZE=350|CAPTION= <scene name='initialview01'>1dw5</scene>
+The line below this paragraph, containing "STRUCTURE_1dw5", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1dw5|  PDB=1dw5  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dw5 OCA], [http://www.ebi.ac.uk/pdbsum/1dw5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dw5 RCSB]</span>
-}}
 '''NMR STRUCTURE OF OMEGA-CONOTOXIN MVIIA: NO CONSTRAINTS ON DISULPHIDE BRIDGES'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-DW5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DW5 OCA].
+DW5 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DW5 OCA].
 ==Reference==
@@ Line 29: / Line 26: @@
 [[Category: Lefevre, J F.]]
 [[Category: Sirockin, F.]]
-[[Category: calcium channel]]
+[[Category: Calcium channel]]
-[[Category: conformational exchange]]
+[[Category: Conformational exchange]]
-[[Category: conotoxin]]
+[[Category: Conotoxin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:21:18 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:49:58 2008''