1dw2: Difference between revisions

Revision as of 14:21, 2 May 2008

STRUCTURE OF THE NITRIC OXIDE COMPLEX OF REDUCED SHP, AN OXYGEN BINDING CYTOCHROME C

OverviewOverview

The photosynthetic bacterium Rhodobacter sphaeroides produces a heme protein (SHP), which is an unusual c-type cytochrome capable of transiently binding oxygen during autooxidation. Similar proteins have not only been observed in other photosynthetic bacteria but also in the obligate methylotroph Methylophilus methylotrophus and the metal reducing bacterium Shewanella putrefaciens. A three-dimensional structure of SHP was derived using the multiple isomorphous replacement phasing method. Besides a model for the oxidized state (to 1.82 A resolution), models for the reduced state (2.1 A resolution), the oxidized molecule liganded with cyanide (1. 90 A resolution), and the reduced molecule liganded with nitric oxide (2.20 A resolution) could be derived. The SHP structure represents a new variation of the class I cytochrome c fold. The oxidized state reveals a novel sixth heme ligand, Asn(88), which moves away from the iron upon reduction or when small molecules bind. The distal side of the heme has a striking resemblance to other heme proteins that bind gaseous compounds. In SHP the liberated amide group of Asn(88) stabilizes solvent-shielded ligands through a hydrogen bond.

About this StructureAbout this Structure

1DW2 is a Single protein structure of sequence from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of an oxygen-binding cytochrome c from Rhodobacter sphaeroides., Leys D, Backers K, Meyer TE, Hagen WR, Cusanovich MA, Van Beeumen JJ, J Biol Chem. 2000 May 26;275(21):16050-6. PMID:10821858 Page seeded by OCA on Fri May 2 14:21:05 2008

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OCA

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 [[Image:1dw2.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dw2 OCA], [http://www.ebi.ac.uk/pdbsum/1dw2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dw2 RCSB]</span>
-}}
 '''STRUCTURE OF THE NITRIC OXIDE COMPLEX OF REDUCED SHP, AN OXYGEN BINDING CYTOCHROME C'''
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 [[Category: Leys, D.]]
 [[Category: Meyer, T E.]]
-[[Category: asparagine ligation]]
+[[Category: Asparagine ligation]]
-[[Category: cytochrome c]]
+[[Category: Cytochrome c]]
-[[Category: disulfide bridge]]
+[[Category: Disulfide bridge]]
-[[Category: nitric oxide]]
+[[Category: Nitric oxide]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:21:05 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:49:53 2008''