1e6g: Difference between revisions
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==A-SPECTRIN SH3 DOMAIN A11V, V23L, M25I, V53I, V58L MUTANT== | ==A-SPECTRIN SH3 DOMAIN A11V, V23L, M25I, V53I, V58L MUTANT== | ||
<StructureSection load='1e6g' size='340' side='right' caption='[[1e6g]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='1e6g' size='340' side='right' caption='[[1e6g]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1shg|1shg]], [[1tuc|1tuc]], [[1tud|1tud]], [[1cun|1cun]], [[1bk2|1bk2]], [[1aj3|1aj3]], [[1aey|1aey]], [[1e6h|1e6h]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1shg|1shg]], [[1tuc|1tuc]], [[1tud|1tud]], [[1cun|1cun]], [[1bk2|1bk2]], [[1aj3|1aj3]], [[1aey|1aey]], [[1e6h|1e6h]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e6g OCA], [http://pdbe.org/1e6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e6g RCSB], [http://www.ebi.ac.uk/pdbsum/1e6g PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e6g OCA], [http://pdbe.org/1e6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e6g RCSB], [http://www.ebi.ac.uk/pdbsum/1e6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e6g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e6/1e6g_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e6/1e6g_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1e6g" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1e6g" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 11:02, 13 December 2017
A-SPECTRIN SH3 DOMAIN A11V, V23L, M25I, V53I, V58L MUTANTA-SPECTRIN SH3 DOMAIN A11V, V23L, M25I, V53I, V58L MUTANT
Structural highlights
Function[SPTN1_CHICK] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedWe have designed de novo 13 divergent spectrin SH3 core sequences to determine their folding properties. Kinetic analysis of the variants with stability similar to that of the wild type protein shows accelerated unfolding and refolding rates compatible with a preferential stabilization of the transition state. This is most likely caused by conformational strain in the native state, as deletion of a methyl group (Ile-->Val) leads to deceleration in unfolding and increased stability (up to 2 kcal x mol(-1)). Several of these Ile-->Val mutants have negative phi(-U) values, indicating that some noncanonical phi(-U) values might result from conformational strain. Thus, producing a stable protein does not necessarily mean that the design process has been entirely successful. Strained interactions could have been introduced, and a reduction in the buried volume could result in a large increase in stability and a reduction in unfolding rates. Conformational strain in the hydrophobic core and its implications for protein folding and design.,Ventura S, Vega MC, Lacroix E, Angrand I, Spagnolo L, Serrano L Nat Struct Biol. 2002 Jun;9(6):485-93. PMID:12006985[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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