2oxt: Difference between revisions
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==Crystal structure of Meaban virus nucleoside-2'-O-methyltransferase== | ==Crystal structure of Meaban virus nucleoside-2'-O-methyltransferase== | ||
<StructureSection load='2oxt' size='340' side='right' caption='[[2oxt]], [[Resolution|resolution]] 2.90Å' scene=''> | <StructureSection load='2oxt' size='340' side='right' caption='[[2oxt]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
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<table><tr><td colspan='2'>[[2oxt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Flavivirus_meaban Flavivirus meaban]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OXT FirstGlance]. <br> | <table><tr><td colspan='2'>[[2oxt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Flavivirus_meaban Flavivirus meaban]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OXT FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oxt OCA], [http://pdbe.org/2oxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2oxt RCSB], [http://www.ebi.ac.uk/pdbsum/2oxt PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oxt OCA], [http://pdbe.org/2oxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2oxt RCSB], [http://www.ebi.ac.uk/pdbsum/2oxt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2oxt ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/2oxt_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/2oxt_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
Revision as of 09:58, 22 August 2018
Crystal structure of Meaban virus nucleoside-2'-O-methyltransferaseCrystal structure of Meaban virus nucleoside-2'-O-methyltransferase
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedViral methyltransferases are involved in the mRNA capping process, resulting in the transfer of a methyl group from S-adenosyl-L-methionine to capped RNA. Two groups of methyltransferases (MTases) are known: (guanine-N7)-methyltransferases (N7MTases), adding a methyl group onto the N7 atom of guanine, and (nucleoside-2'-O-)-methyltransferases (2'OMTases), adding a methyl group to a ribose hydroxyl. We have expressed and purified two constructs of Meaban virus (MV; genus Flavivirus) NS5 protein MTase domain (residues 1-265 and 1-293, respectively). We report here the three-dimensional structure of the shorter MTase construct in complex with the cofactor S-adenosyl-L-methionine, at 2.9 angstroms resolution. Inspection of the refined crystal structure, which highlights structural conservation of specific active site residues, together with sequence analysis and structural comparison with Dengue virus 2'OMTase, suggests that the crystallized enzyme belongs to the 2'OMTase subgroup. Enzymatic assays show that the short MV MTase construct is inactive, but the longer construct expressed can transfer a methyl group to the ribose 2'O atom of a short GpppAC(5) substrate. West Nile virus MTase domain has been recently shown to display both N7 and 2'O MTase activity on a capped RNA substrate comprising the 5'-terminal 190 nt of the West Nile virus genome. The lack of N7 MTase activity here reported for MV MTase may be related either to the small size of the capped RNA substrate, to its sequence, or to different structural properties of the C-terminal regions of West Nile virus and MV MTase-domains. Structural bases for substrate recognition and activity in Meaban virus nucleoside-2'-O-methyltransferase.,Mastrangelo E, Bollati M, Milani M, Selisko B, Peyrane F, Canard B, Grard G, de Lamballerie X, Bolognesi M Protein Sci. 2007 Jun;16(6):1133-45. Epub 2007 May 1. PMID:17473012[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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