1daa: Difference between revisions

Revision as of 13:37, 2 May 2008

CRYSTALLOGRAPHIC STRUCTURE OF D-AMINO ACID AMINOTRANSFERASE COMPLEXED WITH PYRIDOXAL-5'-PHOSPHATE

OverviewOverview

The three-dimensional structure of D-amino acid aminotransferase (D-AAT) in the pyridoxamine phosphate form has been determined crystallographically. The fold of this pyridoxal phosphate (PLP)-containing enzyme is completely different from those of any of the other enzymes that utilize PLP as part of their mechanism and whose structures are known. However, there are some striking similarities between the active sites of D-AAT and the corresponding enzyme that transaminates L-amino acids, L-aspartate aminotransferase. These similarities represent convergent evolution to a common solution of the problem of enforcing transamination chemistry on the PLP cofactor. Implications of these similarities are discussed in terms of their possible roles in the stabilization of intermediates of a transamination reaction. In addition, sequence similarity between D-AAT and branched chain L-amino acid aminotransferase suggests that this latter enzyme will also have a fold similar to that of D-AAT.

About this StructureAbout this Structure

1DAA is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a D-amino acid aminotransferase: how the protein controls stereoselectivity., Sugio S, Petsko GA, Manning JM, Soda K, Ringe D, Biochemistry. 1995 Aug 1;34(30):9661-9. PMID:7626635 Page seeded by OCA on Fri May 2 13:37:19 2008

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OCA

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 [[Image:1daa.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1daa |SIZE=350|CAPTION= <scene name='initialview01'>1daa</scene>, resolution 1.94&Aring;
+The line below this paragraph, containing "STRUCTURE_1daa", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/D-amino-acid_transaminase D-amino-acid transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.21 2.6.1.21] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1daa|  PDB=1daa  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1daa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1daa OCA], [http://www.ebi.ac.uk/pdbsum/1daa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1daa RCSB]</span>
-}}
 '''CRYSTALLOGRAPHIC STRUCTURE OF D-AMINO ACID AMINOTRANSFERASE COMPLEXED WITH PYRIDOXAL-5'-PHOSPHATE'''
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 [[Category: Ringe, D.]]
 [[Category: Sugio, S.]]
-[[Category: aminotransferase]]
+[[Category: Aminotransferase]]
-[[Category: d-alanine]]
+[[Category: D-alanine]]
-[[Category: d-amino acid]]
+[[Category: D-amino acid]]
-[[Category: pyridoxal phosphate]]
+[[Category: Pyridoxal phosphate]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:37:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:37:42 2008''