1d7o: Difference between revisions

Revision as of 13:32, 2 May 2008

CRYSTAL STRUCTURE OF BRASSICA NAPUS ENOYL ACYL CARRIER PROTEIN REDUCTASE COMPLEXED WITH NAD AND TRICLOSAN

OverviewOverview

Molecular genetic studies with strains of Escherichia coli resistant to triclosan, an ingredient of many anti-bacterial household goods, have suggested that this compound works by acting as an inhibitor of enoyl reductase (ENR) and thereby blocking lipid biosynthesis. We present structural analyses correlated with inhibition data, on the complexes of E. coli and Brassica napus ENR with triclosan and NAD(+) which reveal how triclosan acts as a site-directed, picomolar inhibitor of the enzyme by mimicking its natural substrate. Elements of both the protein and the nucleotide cofactor play important roles in triclosan recognition, providing an explanation for the factors controlling its tight binding to the enzyme and for the emergence of triclosan resistance.

About this StructureAbout this Structure

1D7O is a Single protein structure of sequence from Brassica napus. Full crystallographic information is available from OCA.

ReferenceReference

Crystallographic analysis of triclosan bound to enoyl reductase., Roujeinikova A, Levy CW, Rowsell S, Sedelnikova S, Baker PJ, Minshull CA, Mistry A, Colls JG, Camble R, Stuitje AR, Slabas AR, Rafferty JB, Pauptit RA, Viner R, Rice DW, J Mol Biol. 1999 Nov 26;294(2):527-35. PMID:10610777 Page seeded by OCA on Fri May 2 13:32:22 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1d7o.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1d7o |SIZE=350|CAPTION= <scene name='initialview01'>1d7o</scene>, resolution 1.9&Aring;
+The line below this paragraph, containing "STRUCTURE_1d7o", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=TCL:TRICLOSAN'>TCL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1d7o|  PDB=1d7o  |  SCENE=  }}
-|RELATEDENTRY=[[1eno|1ENO]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d7o OCA], [http://www.ebi.ac.uk/pdbsum/1d7o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d7o RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF BRASSICA NAPUS ENOYL ACYL CARRIER PROTEIN REDUCTASE COMPLEXED WITH NAD AND TRICLOSAN'''
@@ Line 24: / Line 21: @@
 Crystallographic analysis of triclosan bound to enoyl reductase., Roujeinikova A, Levy CW, Rowsell S, Sedelnikova S, Baker PJ, Minshull CA, Mistry A, Colls JG, Camble R, Stuitje AR, Slabas AR, Rafferty JB, Pauptit RA, Viner R, Rice DW, J Mol Biol. 1999 Nov 26;294(2):527-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10610777 10610777]
 [[Category: Brassica napus]]
-[[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
 [[Category: Single protein]]
 [[Category: Baker, P J.]]
@@ Line 41: / Line 37: @@
 [[Category: Stuitje, A R.]]
 [[Category: Viner, R]]
-[[Category: enoyl reductase]]
+[[Category: Enoyl reductase]]
-[[Category: triclosan]]
+[[Category: Triclosan]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:32:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:36:28 2008''